Effect of smooth muscle cells on the mechanical response of rabbit carotid arteries in culture

Takeo Matsumoto, Eijiro Okumura, Yuichiro Miura, Masaaki Sato

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The effects of smooth muscle cells on the mechanical response of arterial walls were investigated for the common carotid arteries in culture. Tubular segments obtained from male Japanese White rabbits were cultured for 6 days in the unloaded state in DMEM+20% FBS. Prior to culture, half of the specimens were soaked in liquid nitrogen (liq-N2) for 20 - 40 seconds to kill cells in their walls. The mechanical response of the two groups following culture was examined using the pressure-diameter test and morphometry of the cultured segments. The arterial segments cultured without liq-N2 treatment had significantly smaller outer diameter than their uncultured counterparts over 80 mmHg (P<0.02), and also their wall thickness was significantly reduced (P<0.005). These changes were not significant for the segments treated with liq-N2. These results suggest that mechanical response observed in the cultured arteries are attributable to active remodelling of arterial wall caused by smooth muscle cells.

Original languageEnglish
Pages (from-to)514-520
Number of pages7
JournalJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
Volume42
Issue number3
DOIs
Publication statusPublished - 1999 Sep

Keywords

  • Biomechanics
  • Blood Vessel Wall
  • Elasticity
  • Material Testing
  • Mechanical Adaptation
  • Smooth Muscle
  • Tissue Culture

ASJC Scopus subject areas

  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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