TY - JOUR
T1 - Effect of single-oocyte culture system on in vitro maturation and developmental competence in mice
AU - Nishio, Manami
AU - Hoshino, Yumi
AU - Tanemura, Kentaro
AU - Sato, Eimei
N1 - Funding Information:
Acknowledgments This work was supported by a grant from the Japan Society for the Promotion of Science to E. Sato (Grant No. 21248032). This work was also supported in part by a Grant-in-Aid for Young Scientists (B) to Y. Hoshino (No. 21780250) from the Ministry of Education, Science and Culture, Japan, and by research funding from the Kanzawa Medical Research Foundation to Y. Hoshino.
PY - 2014/7
Y1 - 2014/7
N2 - Purpose: To investigate whether single-culture systems influence the quality of in vitro-matured oocytes, we examined the maturation and developmental competence of oocytes obtained by grouped in vitro maturation (IVM) or single IVM. Methods: In vitro-matured oocytes were obtained using the culture drop (CD) method for the grouped IVM experiments, and the CD and hanging drop (HD) method for the single IVM experiments. To evaluate oocyte developmental competence, we performed in vitro fertilization and culture, and counted the number of blastocysts. To evaluate the oocyte cytoplasmic maturation, we measured the maturation promoting factor (MPF) expression levels. Results: Oocytes cultured singly had lower maturity and developmental competence than the grouped IVM oocytes. However, enhanced oocyte fertility and blastocyst quality was achieved by the HD single IVM method. Additionally, the MPF activity level increased in all culture methods, compared to the control; however, it lagged behind nuclear maturation. Conclusions: These results suggest that the HD method is efficient for single IVM.
AB - Purpose: To investigate whether single-culture systems influence the quality of in vitro-matured oocytes, we examined the maturation and developmental competence of oocytes obtained by grouped in vitro maturation (IVM) or single IVM. Methods: In vitro-matured oocytes were obtained using the culture drop (CD) method for the grouped IVM experiments, and the CD and hanging drop (HD) method for the single IVM experiments. To evaluate oocyte developmental competence, we performed in vitro fertilization and culture, and counted the number of blastocysts. To evaluate the oocyte cytoplasmic maturation, we measured the maturation promoting factor (MPF) expression levels. Results: Oocytes cultured singly had lower maturity and developmental competence than the grouped IVM oocytes. However, enhanced oocyte fertility and blastocyst quality was achieved by the HD single IVM method. Additionally, the MPF activity level increased in all culture methods, compared to the control; however, it lagged behind nuclear maturation. Conclusions: These results suggest that the HD method is efficient for single IVM.
KW - Hanging drop
KW - In vitro maturation
KW - Mouse oocyte
KW - Oocyte quality
KW - Single culture
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U2 - 10.1007/s12522-014-0177-1
DO - 10.1007/s12522-014-0177-1
M3 - Article
AN - SCOPUS:84904737788
VL - 13
SP - 153
EP - 159
JO - Reproductive Medicine and Biology
JF - Reproductive Medicine and Biology
SN - 1445-5781
IS - 3
ER -