Effect of silicon deficiency on secondary cell wall synthesis in rice leaf

Tsuyoshi Yamamoto, Atsuko Nakamura, Hiroaki Iwai, Tadashi Ishii, Jian Feng Ma, Ryusuke Yokoyama, Kazuhiko Nishitani, Shinobu Satoh, Jun Furukawa

    Research output: Contribution to journalArticlepeer-review

    34 Citations (Scopus)

    Abstract

    Rice (Oryza sativa L.) is a typical Si-accumulating plant and is able to accumulate Si up to >10 % of shoot dry weight. The cell wall has been reported to become thicker under Si-deficient condition. To clarify the relationship between Si accumulation and cell wall components, the physical properties of, and macromolecular components and Si content in, the pectic, hemicellulosic, and cellulosic fractions prepared from rice seedlings grown in hydroponics with or without 1. 5 mM silicic acid were analyzed. In the absence of Si (the -Si condition), leaf blades drooped, but physical properties were enhanced. Sugar content in the cellulosic fraction and lignin content in the total cell wall increased under -Si condition. After histochemical staining, there was an increase in cellulose deposition in short cells and the cell layer just beneath the epidermis in the -Si condition, but no significant change in the pattern of lignin deposition. Expression of the genes involved in secondary cell wall synthesis, OsCesA4, OsCesA7, OsPAL, OsCCR1 and OsCAD6 was up-regulated under -Si condition, but expression of OsCesA1, involved in primary cell wall synthesis, did not increase. These results suggest that an increase in secondary cell wall components occurs in rice leaves to compensate for Si deficiency.

    Original languageEnglish
    Pages (from-to)771-779
    Number of pages9
    JournalJournal of Plant Research
    Volume125
    Issue number6
    DOIs
    Publication statusPublished - 2012 Nov

    Keywords

    • Cellulose
    • Lignin
    • Rice
    • Secondary cell wall synthesis
    • Silicon

    ASJC Scopus subject areas

    • Plant Science

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