Effect of shear stress on permeability of vascular endothelial monolayer cocultured with smooth muscle cells

Naoya Sakamoto, Toshiro Ohashi, Masaaki Sato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Effect of fluid shear stress on permeability of endothelial monolayer was investigated using an endothelial cell (EC) - smooth muscle cell (SMC) cocultured model (CM). Permeability of ECs to bovine serum albumin was measured after exposure to shear stress of 1.5 Pa for 48 hours. Morphology and VE-cadherin expression of ECs in CM was almost same as of ECs cultured alone (monocultured model, MM). Under static condition, EC permeability was 5.1 ± 3.0 × 10-6 cm/sec (mean ± SD) in MM and 6.5 ± 3.4 × 10-6 cm/sec in CM. After exposure to shear stress, EC permeability in CM (2.2 ± 1.9 × 10-6 cm/sec, p < 0.05) significantly decreased compared with the static model. However, EC permeability in MM (3.9 ± 3.2 × 10-6 cm/sec) did not significantly change compared with static cultured condition. These results suggested that cellular interactions between ECs and SMCs have important influences on EC permeability.

Original languageEnglish
Pages (from-to)992-999
Number of pages8
JournalJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
Volume47
Issue number4
DOIs
Publication statusPublished - 2004 Dec 1

Keywords

  • Bio-flouid mechanics
  • Biomechanics
  • Cocultured model
  • Morphology
  • Permeability
  • Shear flow
  • VE-cadherin

ASJC Scopus subject areas

  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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