Abstract
Effect of fluid shear stress on permeability of endothelial monolayer was investigated using an endothelial cell (EC) - smooth muscle cell (SMC) cocultured model (CM). Permeability of ECs to bovine serum albumin was measured after exposure to shear stress of 1.5 Pa for 48 hours. Morphology and VE-cadherin expression of ECs in CM was almost same as of ECs cultured alone (monocultured model, MM). Under static condition, EC permeability was 5.1 ± 3.0 × 10-6 cm/sec (mean ± SD) in MM and 6.5 ± 3.4 × 10-6 cm/sec in CM. After exposure to shear stress, EC permeability in CM (2.2 ± 1.9 × 10-6 cm/sec, p < 0.05) significantly decreased compared with the static model. However, EC permeability in MM (3.9 ± 3.2 × 10-6 cm/sec) did not significantly change compared with static cultured condition. These results suggested that cellular interactions between ECs and SMCs have important influences on EC permeability.
| Original language | English |
|---|---|
| Pages (from-to) | 992-999 |
| Number of pages | 8 |
| Journal | JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing |
| Volume | 47 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2004 Dec |
Keywords
- Bio-flouid mechanics
- Biomechanics
- Cocultured model
- Morphology
- Permeability
- Shear flow
- VE-cadherin
ASJC Scopus subject areas
- Mechanical Engineering
- Industrial and Manufacturing Engineering