Effect of Scavenging Circulating Reactive Carbonyls by Oral Pyridoxamine in Uremic Rats on Peritoneal Dialysis

Yoshitaka Mori, Takatoshi Kakuta, Takayo Miyakogawa, Susumu Takekoshi, Hiroko Yuzawa, Hiroyuki Kobayashi, Atsushi Kawakami, Toshio Miyata, Masafumi Fukagawa

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1 Citation (Scopus)

Abstract

Pyridoxamine, a reactive carbonyl (RCO) scavenger, can ameliorate peritoneal deterioration in uremic peritoneal dialysis (PD) rats when given via dialysate. We examined the effects of scavenging circulating RCOs by oral pyridoxamine. Rats underwent nephrectomy and 3 weeks of twice daily PD either alone or with once daily oral pyridoxamine. PD solution was supplemented with methylglyoxal, a major glucose-derived RCO, to quench intraperitoneal pyridoxamine. Oral pyridoxamine achieved comparable blood and dialysate pyridoxamine concentrations, suppressed pentosidine accumulation in the blood but not in the mesenterium or dialysate, and reduced the increases in small solute transport and mesenteric vessel densities, with no effects on submesothelial matrix layer thickening or serum creatinine. Thus, reducing circulating RCOs by giving oral pyridoxamine with PD provides limited peritoneal protection. However, orally given pyridoxamine efficiently reaches the peritoneal cavity and would eliminate intraperitoneal RCOs. Oral pyridoxamine is more clinically favorable and may be as protective as intraperitoneal administration.

Original languageEnglish
Pages (from-to)645-654
Number of pages10
JournalTherapeutic Apheresis and Dialysis
Volume20
Issue number6
DOIs
Publication statusPublished - 2016 Dec 1

Keywords

  • Carbonyl stress
  • Glucose degradation product
  • Mesothelium
  • Neovascularization
  • Pentosidine
  • Peritoneal dialysis
  • Peritoneum

ASJC Scopus subject areas

  • Hematology
  • Nephrology

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  • Cite this

    Mori, Y., Kakuta, T., Miyakogawa, T., Takekoshi, S., Yuzawa, H., Kobayashi, H., Kawakami, A., Miyata, T., & Fukagawa, M. (2016). Effect of Scavenging Circulating Reactive Carbonyls by Oral Pyridoxamine in Uremic Rats on Peritoneal Dialysis. Therapeutic Apheresis and Dialysis, 20(6), 645-654. https://doi.org/10.1111/1744-9987.12446