Effect of recombinant bovine tumor necrosis factor-α on hormone release in lactating cows

Shiro Kushibiki, Hiroyuki Shingu, Tokushi Komatsu, Fumiaki Itoh, Etsuko Kasuya, Hisashi Aso, Koichi Hodate

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    5 Citations (Scopus)


    Tumor necrosis factor (TNF)-α is a powerful macrophage cytokine released during infection, circulating in the blood to produce diverse effects in the organism. We examined the effect of recombinant bovine TNF-α (rbTNF-α) administration on hormone release in dairy cows during early lactation. Twelve non-pregnant Holstein cows were treated subcutaneously with rbTNF-a (2.5 μg/kg) or saline twice (at 11.00 and 23.00 hours). At 11.00 hours the next day, the cows were given growth hormone-releasing hormone (GHRH, 0.25p.g/kg), thyrotrophin-releasing hormone (TRH, 1.0 μg/kg), thyroid-stimulating hormone (TSH, 10 μg/kg) or adrenocorticotropic hormone (500 μg/head) via the jugular vein. In the growth hormone-releasing hormone challenge, the plasma growth hormone concentration was lower in the rbTNF-a group than in the control (saline) group. The growth hormone and TSH responses to TRH were also smaller in the rbTNF-α group than in the control. The plasma prolactin response to TRH was not affected by the rbTNF-α treatment. In the TSH challenge, the rbTNF-α-treated cows had lower responses, as measured by plasma triiodothyronine and thyroxine, than the control cows. The rbTNF-α treatment produced an increase in the basal plasma cortisol level, but the cortisol response to adrenocorticotropic hormone was the same level in both groups. The plasma concentrations of TNF-α and interleukin-1βin the cows were elevated by the rbTNF-α treatment. The milk yield was reduced by the rbTNF-α administration during 4 days. These data demonstrate that TNF-α alters the secretion of pituitary and thyroid hormones in lactating cows. This effect may contribute to the suppression of the lactogenic function of the mammary gland observed in cases of coliform mastitis with high circulating TNF-α levels.

    Original languageEnglish
    Pages (from-to)603-612
    Number of pages10
    JournalAnimal Science Journal
    Issue number6
    Publication statusPublished - 2006 Dec 1


    • Hormone
    • Lactating cow
    • Mastitis
    • Tumor necrosis factor-α

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)


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