Effect of recombinant basic fibroblast growth factor (bFGF) on fibroblast-like cells from human rotator cuff tendon

Shu Takahashi; Mizuho Nakajima; Moto Kobayashi; Ikuko Wakabayashi; Naohisa Miyakoshi; Hiroshi Minagawa; Eiji Itoi

doi:10.1620/tjem.198.207

Effect of recombinant basic fibroblast growth factor (bFGF) on fibroblast-like cells from human rotator cuff tendon

Shu Takahashi, Mizuho Nakajima, Moto Kobayashi, Ikuko Wakabayashi, Naohisa Miyakoshi, Hiroshi Minagawa, Eiji Itoi

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Rotator cuff tendon cells (RCC) derived from surgical samples showed fibroblast-like morphology. Histological staining demonstrated collagen secretion by RCC. Immunohistological findings revealed that RCC secreted type I and III collagen, but not type II collagen. In addition, the SDS-PAGE analysis suggested that RCC predominantly produced type I collagen. Basic fibroblast growth factor (bFGF) had a stimulatory effect on the proliferation of RCC dose-dependently up to 1 ng/ml. Administration of bFGF suppressed the secretion of collagens from RCC in a dose-dependent manner.

Original language	English
Pages (from-to)	207-214
Number of pages	8
Journal	Tohoku Journal of Experimental Medicine
Volume	198
Issue number	4
DOIs	https://doi.org/10.1620/tjem.198.207
Publication status	Published - 2002 Dec 1

Keywords

Basic fibroblast growth factor
Collagen
Primary culture
Proliferation
Rotator cuff tendon

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Effect of recombinant basic fibroblast growth factor (bFGF) on fibroblast-like cells from human rotator cuff tendon. / Takahashi, Shu; Nakajima, Mizuho; Kobayashi, Moto; Wakabayashi, Ikuko; Miyakoshi, Naohisa; Minagawa, Hiroshi; Itoi, Eiji.

In: Tohoku Journal of Experimental Medicine, Vol. 198, No. 4, 01.12.2002, p. 207-214.

Research output: Contribution to journal › Article › peer-review

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