Effect of recombinant basic fibroblast growth factor (bFGF) on fibroblast-like cells from human rotator cuff tendon

Shu Takahashi, Mizuho Nakajima, Moto Kobayashi, Ikuko Wakabayashi, Naohisa Miyakoshi, Hiroshi Minagawa, Eiji Itoi

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Rotator cuff tendon cells (RCC) derived from surgical samples showed fibroblast-like morphology. Histological staining demonstrated collagen secretion by RCC. Immunohistological findings revealed that RCC secreted type I and III collagen, but not type II collagen. In addition, the SDS-PAGE analysis suggested that RCC predominantly produced type I collagen. Basic fibroblast growth factor (bFGF) had a stimulatory effect on the proliferation of RCC dose-dependently up to 1 ng/ml. Administration of bFGF suppressed the secretion of collagens from RCC in a dose-dependent manner.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalTohoku Journal of Experimental Medicine
Volume198
Issue number4
DOIs
Publication statusPublished - 2002 Dec 1

Keywords

  • Basic fibroblast growth factor
  • Collagen
  • Primary culture
  • Proliferation
  • Rotator cuff tendon

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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