Effect of preinductin of metallothionein synthesis on clastogenicity of anticancer drugs in mice

Ippei Nakagaw, Emiko Nishi, Akira Naganuma, Nobumasa Imura

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25 Citations (Scopus)


The effect of pretreatment with metallothionein (MT) inducers (bismuth nitrate or zinc chloride) on clastogenicity of anticancer drugs was investigated. Bismuth nitrate (50 μmol/kg) or zinc chloride (400 μmol/kg) was administered s.c. to mice once a day for two days prior to treatment with 3.3 μmol/kg of cis-diamminedichloroplatinum(II) (cis-DDP), 3.4 μmol/kg of adriamycin (ADR), 72 μmol/kg of cyclophosphamide (CPA) or 0.41 μmol/kg of L-phenylalanine mustard (L-PAM). The frequency of occurrence of erythrocytes with micronuclei in bone marrow was increased by each anticancer drug at 24 h after treatment. Micronucleus formation was significantly prevented by pretreatment with either bismuth nitrate or zinc chloride. MT concentration in bone marrow cells of mice at the time of treatment with anticancer drugs increased to 2- and 3.5-fold by pretreatment with bismuth nitrate and zinc chloride, respectively. These results indicate that MT induction in bone marrow cells effectively prevents micronucleus induction of anticancer drugs.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalMutation Research Letters
Issue number1
Publication statusPublished - 1995 Sep
Externally publishedYes


  • Adriamycin
  • Bismuth
  • Cyclophosphamide
  • L-Phenylalanine mustard
  • Metallothionein
  • Micronucleus test
  • Zinc
  • cis-DDP

ASJC Scopus subject areas

  • Medicine(all)


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