Effect of pore size of self-organized honeycomb-patterned polymer films on spreading, focal adhesion, proliferation, and function of endothelial cells

Masaru Tanaka, Aiko Takayama, Emiko Ito, Hiroshi Sunami, Sadaaki Yamamoto, Masatsugu Shimomura

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    The design of nano- and microstructures based on self-organization is a key area of research in the search for new materials, and it has a variety of potential applications in tissue engineering scaffolds. We have reported a honeycomb-patterned polymer film (honeycomb film) with highly regular pores that is formed by self-organization. This study describes the behavior of vascular endothelial cells (ECs) on honeycomb films with four different pore sizes (5, 9, 12, and 16 μm) as well as on a flat film. We examined the influence of the honeycomb pattern and pore size on cell behavior. The changes in cell morphologies, actin filaments, vinculin clusters, cell proliferation, and secreted extracellular matrix (ECM) (fibronectin, laminin, type IV collagen, and elastin) production profiles were observed by using optical, fluorescence, and scanning electron microscopy. The ECs that adhered to the flat film showed an elongated morphology with random orientation; the actin filaments and focal adhesions were not conspicuous. On the other hand, the ECs on the honeycomb films exhibited greater spreading and flattening; the degree of spreading of the ECs increased with an increase in the pore size. The actin filaments and focal adhesions appeared conspicuous, and the focal adhesions localized along the edge of the honeycomb pores were distributed over the entire projected cell area. The honeycomb film with a pore size of 5 μm showed the highest cell proliferation and ECM production profiles. These results suggest that the honeycomb film is a suitable material for designing a new vascular device.

    Original languageEnglish
    Pages (from-to)763-772
    Number of pages10
    JournalJournal of Nanoscience and Nanotechnology
    Volume7
    Issue number3
    DOIs
    Publication statusPublished - 2007 Mar 1

    Keywords

    • Cell adhesion
    • Focal adhesion
    • Pore size
    • Scaffold
    • Self-organization

    ASJC Scopus subject areas

    • Chemistry(all)
    • Materials Science(all)
    • Materials Science (miscellaneous)
    • Engineering (miscellaneous)

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