Effect of pore size of honeycomb films on the morphology, adhesion and cytoskeletal organization of cardiac myocytes

Keiko Arai, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    Cells respond to the circumstances such as structures and chemical composition of scaffolds and cytokine. In this report, the responses of cytoskeleton and spreading of cardiac myocytes to the self-organized honeycomb-patterned films (honeycomb films) of biodegradable poly(ε-caprolactone) (PCL) with several pore sizes were investigated. The expression of actin filament of cardiac myocytes was strongly dependent on the pore size ranging from 4 to 13 μm. Immunofluorescent labeling of vinculin in cardiac myocytes showed localization of focal contact along the edge of the honeycomb pores. The results indicated that the honeycomb structures and the pore sizes influence the morphology, cytoskeletal organization and focal adhesion of the cardiac myocytes.

    Original languageEnglish
    Pages (from-to)530-535
    Number of pages6
    JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
    Volume313-314
    DOIs
    Publication statusPublished - 2008 Feb 1

    Keywords

    • Cardiac myocytes
    • Cardiac tissue engineering
    • Cell adhesion
    • Cell morphology
    • Honeycomb film
    • Micropatterning
    • Scaffold

    ASJC Scopus subject areas

    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

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