Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics

Naohisa Miyatake; Koshi N. Kishimoto; Takahisa Anada; Hideki Imaizumi; Eiji Itoi; Osamu Suzuki

doi:10.1016/j.biomaterials.2008.10.058

Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics

Naohisa Miyatake, Koshi N. Kishimoto, Takahisa Anada, Hideki Imaizumi, Eiji Itoi, Osamu Suzuki

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

The present study was designed to investigate whether the stoichiometry of octacalcium phosphate OCP affects its osteoconductive and immune response characteristics in rat bone marrow. Those characteristics of synthetic, well-grown OCP but with a non-stoichiometric composition were compared with those of a slightly hydrolyzed OCP (low crystalline OCP: LC-OCP), the fully hydrolyzed apatitic product of OCP or biodegradable β-tricalcium phosphate (β-TCP) ceramic, by their implantation in rat tibia for 56 days. The physicochemical aspect of implants and biological responses were analyzed by X-ray diffraction, histomorphometry, immunohistochemistry and expression of mRNA around the implants. The remarkable findings were that: (1) the highest bone formation rate was obtained for β-TCP whereas the lowest for LC-OCP at Day 14; (2) the rates were reversed and reached the highest for LC-OCP until Day 56; (3) the early expression of ostoeoclast markers TRAP and cathepsin-K was suppressed with LC-OCP; (4) the expression of inflammatory markers IL-β1 and TNF-α was suppressed with LC-OCP. The results confirmed that the partially hydrolyzed OCP with Ca/P molar ratio 1.37 (LC-OCP) enhances bone formation most, suppressing early osteoclast activity and reducing inflammation.

Original language	English
Pages (from-to)	1005-1014
Number of pages	10
Journal	Biomaterials
Volume	30
Issue number	6
DOIs	https://doi.org/10.1016/j.biomaterials.2008.10.058
Publication status	Published - 2009 Feb

Keywords

Biodegradation
Bone marrow
Octacalcium phosphate
Osteconduction
Partial hydrolysis

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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@article{918483173e5447bc91536ca7f27632b6,

title = "Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics",

abstract = "The present study was designed to investigate whether the stoichiometry of octacalcium phosphate OCP affects its osteoconductive and immune response characteristics in rat bone marrow. Those characteristics of synthetic, well-grown OCP but with a non-stoichiometric composition were compared with those of a slightly hydrolyzed OCP (low crystalline OCP: LC-OCP), the fully hydrolyzed apatitic product of OCP or biodegradable β-tricalcium phosphate (β-TCP) ceramic, by their implantation in rat tibia for 56 days. The physicochemical aspect of implants and biological responses were analyzed by X-ray diffraction, histomorphometry, immunohistochemistry and expression of mRNA around the implants. The remarkable findings were that: (1) the highest bone formation rate was obtained for β-TCP whereas the lowest for LC-OCP at Day 14; (2) the rates were reversed and reached the highest for LC-OCP until Day 56; (3) the early expression of ostoeoclast markers TRAP and cathepsin-K was suppressed with LC-OCP; (4) the expression of inflammatory markers IL-β1 and TNF-α was suppressed with LC-OCP. The results confirmed that the partially hydrolyzed OCP with Ca/P molar ratio 1.37 (LC-OCP) enhances bone formation most, suppressing early osteoclast activity and reducing inflammation.",

keywords = "Biodegradation, Bone marrow, Octacalcium phosphate, Osteconduction, Partial hydrolysis",

author = "Naohisa Miyatake and Kishimoto, {Koshi N.} and Takahisa Anada and Hideki Imaizumi and Eiji Itoi and Osamu Suzuki",

note = "Funding Information: This study was supported in part by Grants-in-Aid (17076001, 18659567, 19390490, and 20659304) from the Ministry of Education, Science, Sports and Culture of Japan. The authors thank Shinji Morimoto, Research and Development Laboratory, NIPRO Corp., Kusatsu, Japan, for the analysis of Ca/P molar ratio of OCP and its hydrolyzates. ",

year = "2009",

month = feb,

doi = "10.1016/j.biomaterials.2008.10.058",

language = "English",

volume = "30",

pages = "1005--1014",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "6",

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TY - JOUR

T1 - Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics

AU - Miyatake, Naohisa

AU - Kishimoto, Koshi N.

AU - Anada, Takahisa

AU - Imaizumi, Hideki

AU - Itoi, Eiji

AU - Suzuki, Osamu

N1 - Funding Information: This study was supported in part by Grants-in-Aid (17076001, 18659567, 19390490, and 20659304) from the Ministry of Education, Science, Sports and Culture of Japan. The authors thank Shinji Morimoto, Research and Development Laboratory, NIPRO Corp., Kusatsu, Japan, for the analysis of Ca/P molar ratio of OCP and its hydrolyzates.

PY - 2009/2

Y1 - 2009/2

N2 - The present study was designed to investigate whether the stoichiometry of octacalcium phosphate OCP affects its osteoconductive and immune response characteristics in rat bone marrow. Those characteristics of synthetic, well-grown OCP but with a non-stoichiometric composition were compared with those of a slightly hydrolyzed OCP (low crystalline OCP: LC-OCP), the fully hydrolyzed apatitic product of OCP or biodegradable β-tricalcium phosphate (β-TCP) ceramic, by their implantation in rat tibia for 56 days. The physicochemical aspect of implants and biological responses were analyzed by X-ray diffraction, histomorphometry, immunohistochemistry and expression of mRNA around the implants. The remarkable findings were that: (1) the highest bone formation rate was obtained for β-TCP whereas the lowest for LC-OCP at Day 14; (2) the rates were reversed and reached the highest for LC-OCP until Day 56; (3) the early expression of ostoeoclast markers TRAP and cathepsin-K was suppressed with LC-OCP; (4) the expression of inflammatory markers IL-β1 and TNF-α was suppressed with LC-OCP. The results confirmed that the partially hydrolyzed OCP with Ca/P molar ratio 1.37 (LC-OCP) enhances bone formation most, suppressing early osteoclast activity and reducing inflammation.

AB - The present study was designed to investigate whether the stoichiometry of octacalcium phosphate OCP affects its osteoconductive and immune response characteristics in rat bone marrow. Those characteristics of synthetic, well-grown OCP but with a non-stoichiometric composition were compared with those of a slightly hydrolyzed OCP (low crystalline OCP: LC-OCP), the fully hydrolyzed apatitic product of OCP or biodegradable β-tricalcium phosphate (β-TCP) ceramic, by their implantation in rat tibia for 56 days. The physicochemical aspect of implants and biological responses were analyzed by X-ray diffraction, histomorphometry, immunohistochemistry and expression of mRNA around the implants. The remarkable findings were that: (1) the highest bone formation rate was obtained for β-TCP whereas the lowest for LC-OCP at Day 14; (2) the rates were reversed and reached the highest for LC-OCP until Day 56; (3) the early expression of ostoeoclast markers TRAP and cathepsin-K was suppressed with LC-OCP; (4) the expression of inflammatory markers IL-β1 and TNF-α was suppressed with LC-OCP. The results confirmed that the partially hydrolyzed OCP with Ca/P molar ratio 1.37 (LC-OCP) enhances bone formation most, suppressing early osteoclast activity and reducing inflammation.

KW - Biodegradation

KW - Bone marrow

KW - Octacalcium phosphate

KW - Osteconduction

KW - Partial hydrolysis

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JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

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