Effect of NND-318 on the Ultrastructure of Trichophyton mentagrophytes as Observed by Scanning and Transmission Electron Microscopy

Tetsuto Ohmi, Sadayoshi Sakumoto, Yoshimi Niwano, Kazuo Kanai, Tetsuji Tanaka, Takashi Suzuki, Matazaemon Uchida

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Effect of NND-318 on the morphology of growing hyphae of Trichophyton mentagrophytes was observed by scanning and transmission electron microscopy. Microconidia of T. mentagrophytes were inoculated on a slide-culture and incubated at 27°C for 60 hours. In the control culture grown without the drug, the hyphae showed a smooth surface, straight extensions and consistent width. In the culture grown with NND-318 at concentrations ranging from 0.08 to 0.4 ng/ml, scanning electron microscopy revealed that hyphae were wavy, curled and bent with wrinkled surface. In ultrathin sections, thickening of the cell wall, accumulation of electron denes granular structures formed within the cell wall, and the number of cytoplasmic lipid droplets were observed to increase with drug concentration. At concentrations ranging from 0.8 to 1.6ng/ml, hyphae were short in length and the surface was heavily wrinkled. At concentrations above 4 ng/ml, germination of inoculated conidia was not observed. Similar morphological changes were seen in T. mentagrophytes treated with higher concentrations of bifonazole. These results suggest that NND-318, at concentrations considerably lower than those of other imidazoles, is able to inhibit hyphal growth of T. mentagrophytes with thickening of cell wall, accumulation of electron dense granules formed in the cell wall and increased number of cytoplasmic lipid droplets.

Original languageEnglish
Pages (from-to)339-348
Number of pages10
JournalNippon Ishinkin Gakkai Zasshi
Volume33
Issue number3
DOIs
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Effect of NND-318 on the Ultrastructure of Trichophyton mentagrophytes as Observed by Scanning and Transmission Electron Microscopy'. Together they form a unique fingerprint.

Cite this