Effect of N,N-diethyldithiocarbamate on ionomycin-induced increase in oxidation of cellular 2′,7′-dichlorofluorescin in dissociated cerebellar neurons

Yasuo Oyama, Katsutoshi Furukawa, Lumi Chikahisa, Yoshiko Hatakeyama

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Effect of N,N-diethyldithiocarbamate (DDC), an inhibitor for cytosolic superoxide dismutase, on an ionomycin-induced increase in oxidative metabolism was examined in cerebellar neurons dissociated from the rats, using a flow cytometer and 2′,7′-dichlorofluorescin diacetate and fluo-3-AM, fluorescent dyes for intracellular hydrogen peroxide and Ca2+, respectively. DDC reduced the ionomycin-induced augmentation of 2′-7′-dichlorofluorescin fluorescence in a dose-dependent manner. DDC did not affect cellular content of 2′,7′-dichlorofluorescin and ionomycin-induced increase in intracellular Ca2+ concentration. Results indicate that ionomycin increases the formation of superoxide anion in brain neuron.

Original languageEnglish
Pages (from-to)158-161
Number of pages4
JournalBrain research
Volume660
Issue number1
DOIs
Publication statusPublished - 1994 Oct 10

Keywords

  • 2′,7′-Dichlorofluorescin diacetate
  • Cerebellar neuron
  • Ionomycin
  • N,N-Diethyldithiocarbamate
  • Oxidative metabolism

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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