Effect of neurotropic virus infection on neuronal and inducible nitric oxide synthase activity in rat brain.

T. Akaike, E. Weihe, M. Schaefer, Z. F. Fu, Y. M. Zheng, W. Vogel, H. Schmidt, H. Koprowski, B. Dietzschold

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84 Citations (Scopus)

Abstract

To elucidate the potential role of inducible nitric oxide synthase (iNOS) and neuronal constitutive nitric oxide synthase (cNOS) in the pathogenesis of virus-induced encephalopathy, the activities of both NOS isoforms were determined in the brains of rats infected with Borna disease virus (BDV) or rabies virus. iNOS activity strongly increased, whereas neuronal cNOS activity significantly decreased in a time-dependent manner after either BDV or rabies virus infection. Choline acetyltransferase activity in the brain remained unchanged during both virus infections, suggesting that the decrease in cNOS activity does not reflect a generalized neuronal loss. Immunohistochemistry and Northern blot analyses indicate that the decrease in neuronal cNOS activity is due to a decrease in cNOS protein and mRNA synthesis. These results suggest that both an excessive generation of NO by activated macrophages or microglia, as well as a decrease of NO production in neurons may contribute to the neuropathogenesis of neurotropic virus infections.

Original languageEnglish
Pages (from-to)118-125
Number of pages8
JournalJournal of neurovirology
Volume1
Issue number1
DOIs
Publication statusPublished - 1995 Mar

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

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    Akaike, T., Weihe, E., Schaefer, M., Fu, Z. F., Zheng, Y. M., Vogel, W., Schmidt, H., Koprowski, H., & Dietzschold, B. (1995). Effect of neurotropic virus infection on neuronal and inducible nitric oxide synthase activity in rat brain. Journal of neurovirology, 1(1), 118-125. https://doi.org/10.3109/13550289509111016