Effect of nanosheet surface structure of titanium alloys on cell differentiation

Satoshi Komasa, Tetsuji Kusumoto, Yoichiro Taguchi, Hiroshi Nishizaki, Tohru Sekino, Makoto Umeda, Joji Okazaki, Takayoshi Kawazoe

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Titanium alloys are the most frequently used dental implants partly because of the protective oxide coating that spontaneously forms on their surface. We fabricated titania nanosheet (TNS) structures on titanium surfaces by NaOH treatment to improve bone differentiation on titanium alloy implants. The cellular response to TNSs on Ti6Al4V alloy was investigated, and the ability of the modified surfaces to affect osteogenic differentiation of rat bone marrow cells and increase the success rate of titanium implants was evaluated. The nanoscale network structures formed by alkali etching markedly enhanced the functions of cell adhesion and osteogenesis-related gene expression of rat bone marrow cells. Other cell behaviors, such as proliferation, alkaline phosphatase activity, osteocalcin deposition, and mineralization, were also markedly increased in TNS-modified Ti6Al4V. Our results suggest that titanium implants modified with nanostructures promote osteogenic differentiation, which may improve the biointegration of these implants into the alveolar bone.

Original languageEnglish
Article number642527
JournalJournal of Nanomaterials
Publication statusPublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)


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