TY - JOUR
T1 - Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold
AU - Yamada, Masakazu
AU - Anada, Takahisa
AU - Masuda, Taisuke
AU - Takano-Yamamoto, Teruko
AU - Suzuki, Osamu
N1 - Funding Information:
This study was supported in part by grants-in-aid ( 23106010 , 25670829 , 26670846 , 26282133 and 26293417 ) from the Ministry of Education, Science, Sports, and Culture of Japan .
Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/6/14
Y1 - 2015/6/14
N2 - We report the extent of mechanical stress on a bioactive three-dimensional scaffold that can induce differentiation of seeded osteoblastic cells in vitro. Mouse mesenchymal stem D1 cells were seeded on a porous spongy scaffold consists of octacalcium phosphate (OCP) and gelatin (Gel) composite, which was previously proven to be a sufficient bone-replaceable material in vivo. The cellular activity was determined after loading of uni-axial compression by deforming a 5 mm ring-shaped, cell-seeded OCP/Gel composite in the range of 1-3 mm (corresponding to 20-60% of the material thickness) using a cyclic mechanical loading device designed for this study. The extent of the deformation did not affect the proliferation. However, enhancement of differentiation marker genes, such as osteopontin and osteoclacin, occurred after the composite was deformed by just 20%. These results suggest that osteoblastic differentiation of D1 cells is promoted if suitable mechanical stress is provided, which indicates biological relevance of the bone regenerative properties of this material under mechanical stress that were shown in a previous in vivo study.
AB - We report the extent of mechanical stress on a bioactive three-dimensional scaffold that can induce differentiation of seeded osteoblastic cells in vitro. Mouse mesenchymal stem D1 cells were seeded on a porous spongy scaffold consists of octacalcium phosphate (OCP) and gelatin (Gel) composite, which was previously proven to be a sufficient bone-replaceable material in vivo. The cellular activity was determined after loading of uni-axial compression by deforming a 5 mm ring-shaped, cell-seeded OCP/Gel composite in the range of 1-3 mm (corresponding to 20-60% of the material thickness) using a cyclic mechanical loading device designed for this study. The extent of the deformation did not affect the proliferation. However, enhancement of differentiation marker genes, such as osteopontin and osteoclacin, occurred after the composite was deformed by just 20%. These results suggest that osteoblastic differentiation of D1 cells is promoted if suitable mechanical stress is provided, which indicates biological relevance of the bone regenerative properties of this material under mechanical stress that were shown in a previous in vivo study.
KW - Gelatin
KW - Mechanical stress
KW - Mesenchymal stem cells
KW - Octacalcium phosphate
KW - Scaffold
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U2 - 10.1016/j.snb.2015.05.073
DO - 10.1016/j.snb.2015.05.073
M3 - Article
AN - SCOPUS:84931266745
VL - 220
SP - 125
EP - 130
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
ER -