Effect of long-term exposure to sulfides on taurine transporter gene expression in the gill of the deep-sea mussel Bathymodiolus platifrons, which harbors a methanotrophic symbiont

Tomoko Koito, Ikumi Nakamura-Kusakabe, Takao Yoshida, Tadashi Maruyama, Tamano Omata, Nobuyuki Miyazaki, Koji Inoue

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Seawater around deep-sea hydrothermal vents and cold seeps contain high levels of hydrogen sulfide, which is toxic to most animals. Invertebrates inhabiting these environments have been reported to accumulate high levels of thiotaurine, a sulfur-containing amino acid. Thiotaurine is likely to play an important role in sulfide detoxification, but its functions in the detoxification process are still unknown. We cloned methane-seep mussel Bathymodiolus platifrons cDNA encoding the taurine transporter (TAUT), which transports thiotaurine and its precursors across the cell membrane. Phylogenetic analyses revealed that the predicted peptide formed a clade with the TAUTs of shallow-water mussel Mytilus galloprovincialis and the hydrothermal vent mussel Bathymodiolus septemdierum that harbors thioautotrophic bacteria. We then reared B. platifrons in the presence or absence of Na2S and quantified TAUT mRNA using a real-time PCR system. The amount of TAUT mRNA in the gills of B. platifrons increased with rearing in the presence of Na2S for 69 days, but no change was observed in the absence of sulfide. These results suggest that TAUT plays an important role in sulfide detoxification, even in species that do not harbor thioautotrophic bacteria. The TAUT mRNA level was variable in the mantle and low in the foot throughout the entire rearing period, regardless of the presence/absence of sulfide, suggesting that TAUT gene expression is regulated differentially in each tissue.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalFisheries Science
Volume76
Issue number2
DOIs
Publication statusPublished - 2010 Mar 1
Externally publishedYes

Keywords

  • Bathymodiolus platifrons
  • Hypotaurine
  • Solute carrier family 6
  • Taurine transporter (TAUT)
  • Thiotaurine

ASJC Scopus subject areas

  • Aquatic Science

Fingerprint Dive into the research topics of 'Effect of long-term exposure to sulfides on taurine transporter gene expression in the gill of the deep-sea mussel Bathymodiolus platifrons, which harbors a methanotrophic symbiont'. Together they form a unique fingerprint.

  • Cite this