We developed an organ culture system in order to examine the effect of intraluminal pressure on intimal thickening in injured arteries. After endothelial denudation, an excised rabbit common carotid artery was incubated at 37°C in a glass bottle perfused with Dulbecco's modified Eagle medium containing 10% fetal calf serum at a constant flow rate of 6 ml/h at various static pressure of 60, 95, 130, 165, or 200 cmH2O. Intimal thickening, which consisted of smooth muscle cells and extracellular matrix, increased in a time- and pressure-dependent manner up to 130 cmH2O. Excessive pressures (165 and 200 cmH2O), however, caused necrosis in the media. These results suggest that a rise in intraluminal pressure accelerates intimal thickening in injured arteries.
- Injured artery
- Intimal thickening
- Organ culture
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine