Effect of intraluminal pressure on the intimal thickening in injured rabbit carotid arteries in an organ-culture system

Hisanori Horiuchi, Masayuki Yokode, Kenji Ishii, Mikimaro Cho, Seijiro Hara, Atsushi Sakai, Kazuo Kanai, Toyohiro Tsukada, Toru Kita

Research output: Contribution to journalArticle

Abstract

We developed an organ culture system in order to examine the effect of intraluminal pressure on intimal thickening in injured arteries. After endothelial denudation, an excised rabbit common carotid artery was incubated at 37°C in a glass bottle perfused with Dulbecco's modified Eagle medium containing 10% fetal calf serum at a constant flow rate of 6 ml/h at various static pressure of 60, 95, 130, 165, or 200 cmH2O. Intimal thickening, which consisted of smooth muscle cells and extracellular matrix, increased in a time- and pressure-dependent manner up to 130 cmH2O. Excessive pressures (165 and 200 cmH2O), however, caused necrosis in the media. These results suggest that a rise in intraluminal pressure accelerates intimal thickening in injured arteries.

Original languageEnglish
Pages (from-to)704-708
Number of pages5
JournalJAPANESE CIRCULATION JOURNAL
Volume61
Issue number8
DOIs
Publication statusPublished - 1997 Sep 12
Externally publishedYes

Keywords

  • Hypertension
  • Injured artery
  • Intimal thickening
  • Organ culture
  • Stretching

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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