Effect of impaired glucose tolerance on atherosclerotic lesion formation: An evaluation in selectively bred mice with different susceptibilities to glucose intolerance

Akira Asai, Mototsugu Nagao, Momoyo Kawahara, Yuki Shuto, Hitoshi Sugihara, Shinichi Oikawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28 weeks of age). Oral glucose tolerance tests were performed during the AD-feeding period. Atherosclerotic lesion formation was quantitatively analyzed in serial aortic sinus sections by oil red O staining. Plasma lipids were measured after the AD-feeding period. Results: Glucose tolerance was impaired in SDG-P mice as compared to SDG-R mice over the 20-week AD-feeding period. No significant differences were observed in any plasma lipid measurement between the 2 mouse lines. Aortic sinus atherosclerotic lesion formation in SDG-P mice was approximately 4-fold greater than that in SDG-R mice. Conclusion: In 2 mouse lines with different susceptibilities to diet-induced glucose intolerance, IGT accelerated atherosclerotic lesion formation. These mice may therefore serve as useful invivo models for investigating the causal role of IGT in the pathogenesis of atherosclerosis.

Original languageEnglish
Pages (from-to)421-426
Number of pages6
JournalAtherosclerosis
Volume231
Issue number2
DOIs
Publication statusPublished - 2013 Dec 1
Externally publishedYes

Keywords

  • Animal model
  • Atherosclerosis
  • Impaired glucose tolerance
  • Postprandial hyperglycemia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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