Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells

Woro Anindito Sri Tunjung, Masaki Yokoo, Yumi Hoshino, Yuko Miyake, Akane Kadowaki, Eimei Sato

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus-oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA-CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA-CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells.

    Original languageEnglish
    Pages (from-to)160-164
    Number of pages5
    JournalBiochemical and biophysical research communications
    Volume382
    Issue number1
    DOIs
    Publication statusPublished - 2009 Apr 24

    Keywords

    • Apoptosis of granulosa cells
    • CD44
    • Caspase
    • FSH
    • Hyaluronan

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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