Abstract
We investigated the effect of herbimycin A on the monolayer growth of 4 human renal cell carcinoma (RCC) cell lines and a normal renal tubular cell line (RTC 13) using MTT [3-(4,5-dimethylthiszol-2,5-diphenyl tetrazolium bromide)] assay. Herbimycin A induced remarkable growth inhibition in each RCC cell line tested, without any morphological changes of the cells. At the concentration of 500 ng/ml, herbimycin A caused more than a 30% growth inhibition in all RCC cells (p<0.005 vs RTC 13), while less than 7% growth inhibition was observed in RTC 13 at the same herbimycin A concentration. The cell cycle was estimated by analysing DNA (deoxyribonucleic acid) content using a FACS. A DNA histogram of RCC cells treated at herbimycin A showed a block in the cell cycle at the S and G2M phases. However, little effect by herbimycin A on RTC 13 cells was observed. Our results suggest that protein tyrosine kinases inhibitors, like herbimycin A, may offer a new treatment option for RCC patients.
| Original language | English |
|---|---|
| Pages (from-to) | 198-201 |
| Number of pages | 4 |
| Journal | Japanese Journal of Nephrology |
| Volume | 38 |
| Issue number | 5 |
| Publication status | Published - 1996 Jul 1 |
| Externally published | Yes |
Keywords
- antiproliferative effect
- herbimycin A
- renal cancer cell
ASJC Scopus subject areas
- Nephrology
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