Effect of herbimycin A on renal cancer cell growth

Akira Tsuji, Tomohiko Asano, Satoshi Suzuki, Teichiro Aoyagi, Masamichi Hayakawa, Hiroshi Nakamura, Toshio Miyata

Research output: Contribution to journalArticle

Abstract

We investigated the effect of herbimycin A on the monolayer growth of 4 human renal cell carcinoma (RCC) cell lines and a normal renal tubular cell line (RTC 13) using MTT [3-(4,5-dimethylthiszol-2,5-diphenyl tetrazolium bromide)] assay. Herbimycin A induced remarkable growth inhibition in each RCC cell line tested, without any morphological changes of the cells. At the concentration of 500 ng/ml, herbimycin A caused more than a 30% growth inhibition in all RCC cells (p<0.005 vs RTC 13), while less than 7% growth inhibition was observed in RTC 13 at the same herbimycin A concentration. The cell cycle was estimated by analysing DNA (deoxyribonucleic acid) content using a FACS. A DNA histogram of RCC cells treated at herbimycin A showed a block in the cell cycle at the S and G2M phases. However, little effect by herbimycin A on RTC 13 cells was observed. Our results suggest that protein tyrosine kinases inhibitors, like herbimycin A, may offer a new treatment option for RCC patients.

Original languageEnglish
Pages (from-to)198-201
Number of pages4
JournalJapanese Journal of Nephrology
Volume38
Issue number5
Publication statusPublished - 1996 Jul 1
Externally publishedYes

Keywords

  • antiproliferative effect
  • herbimycin A
  • renal cancer cell

ASJC Scopus subject areas

  • Nephrology

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  • Cite this

    Tsuji, A., Asano, T., Suzuki, S., Aoyagi, T., Hayakawa, M., Nakamura, H., & Miyata, T. (1996). Effect of herbimycin A on renal cancer cell growth. Japanese Journal of Nephrology, 38(5), 198-201.