Effect of foot shock stress on the interferon-γ production of murine intestinal intraepithelial lymphocytes

Xiumin Zhang, Mitsuharu Okutsu, Osamu Kanemi, Ryoichi Nagatomi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

To investigate the effect of stress on interferon (IFN)-γ production by intestinal intraepithelial lymphocytes (IEL), we exposed male C3H/HeN mice to electric foot shock for 30 min a day for 5 consecutive days. Immediately after the final foot shock stress, IEL from small intestine were isolated by Percoll density gradient. The stress induced a marked suppression of IFN-γ production by IEL stimulated with immobilized anti-CD3 mAb and a marked decrease in the proportion of IFN-γ-producing CD3+ IEL or αβTCR+ IEL stimulated with PMA + ionomycin. The αβTCR+ subset was the major cause of stress-induced suppression of IFN-γ production by IEL. Glucocorticoid induced the suppression of IFN-γ production by IEL in vitro, which was reversed by mifepristone (RU486), a glucocorticoid receptor antagonist. In vivo administration of RU486 reversed the stress-induced suppression of IFN-γ production by IEL. In conclusion, repeated foot shock stress suppressed IFN-γ production of IEL by stress-induced elevation of endogenous glucocorticoid. Substantial suppression of the αβTCR+ subset was the major cause of the suppression.

Original languageEnglish
Pages (from-to)170-176
Number of pages7
JournalImmunology Letters
Volume100
Issue number2
DOIs
Publication statusPublished - 2005 Sep 15
Externally publishedYes

Keywords

  • Glucocorticoid
  • Interferon-γ
  • Intestinal intraepithelial lymphocytes
  • Stress

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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