Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria-material interface: An in vitro model study

Gen Mayanagi, Koei Igarashi, Jumpei Washio, Hitomi Domon-Tawaraya, Nobuhiro Takahashi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Objectives Inhibition of bacterial acid production by dental restorative materials is one of the strategies for secondary caries prevention. This study aimed to evaluate the effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria-material interface. Methods Four fluoride-releasing restorative materials, glass-ionomer cement (GIC), resin-modified glass-ionomer cement (RMGIC), resin composite (RC) and flowable resin composite (FRC) were used. Each specimen was immersed in potassium phosphate buffer at pH 7.0 for 10 min and 4 weeks, and in potassium acetate buffer at pH 5.5 for 4 weeks. An experimental apparatus was made of polymethyl methacrylate and had a well with restorative materials or polymethyl methacrylate (control) at the bottom. The well was packed with cells of Streptococcus mutans, and the pH at the interface between cells and materials was monitored using a miniature pH electrode after the addition of 1% glucose for 90 min, and the fluoride released into the well was quantified using a fluoride ion electrode. Results The pH of GIC (4.98-5.18), RMGIC (4.77-4.99), RC (4.62-4.75) and FRC (4.54-4.84) at 90 min were higher than that of control (4.31-4.49). The fluoride amounts released from GIC were the highest, followed by RMGIC, RC and FRC, irrespective of immersion conditions. Saliva coating on materials had no significant effect. Conclusions The fluoride-releasing restorative materials inhibited pH fall at the bacteria-material interface. The degree of inhibition of pH fall seemed to correspond to the amount of fluoride detected, suggesting that the inhibition was due to the fluoride released from these materials. Clinical significance A little amount of fluoride actually released from the fluoride-releasing materials may have caries preventive potential for oral bacteria.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalJournal of Dentistry
Volume42
Issue number1
DOIs
Publication statusPublished - 2014 Jan

Keywords

  • Fluoride
  • ISFET pH electrode
  • Interface
  • Restorative material
  • Saliva
  • Secondary caries
  • Streptococcus
  • pH

ASJC Scopus subject areas

  • Dentistry(all)

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