Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans

T. Watamoto, L. P. Samaranayake, J. A.M.S. Jayatilake, H. Egusa, H. Yatani, C. J. Seneviratne

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Biofilm formation involving profuse hyphal growth is a major characteristic of Candida spp. and confers higher antifungal resistance than its planktonic mode of growth. We investigated the antifungal susceptibility of Candida albicans and its hyphal mutants (Δefg1/efg1, Δcph1/cph1 and ΔΔcph1/cph1 efg1/efg1) to commonly used antifungals during planktonic, adhesion and biofilm modes of growth. The minimum inhibitory concentration (MIC) of each antifungal agent was determined for a lower inoculum (1 × 103 cells/mL) and higher inoculum (1 × 107 cells/mL) of planktonic Candida. Furthermore, MICs of C. albicans biofilms and adhesion modes of growth were determined with a standard XTT assay. Candida albicans in adhesion and biofilm modes of growth, but not in planktonic mode, were resistant to all five antifungal agents tested. Although Δefg1/efg1 and ΔΔcph1/cph1 efg1/efg1 mutants formed less biofilm than wild-type C. albicans SC5314, they were similarly resistant to caspofungin. However, these mutants were more sensitive to amphotericin B and nystatin than the wild-type. Adhesion per se confers increased resistance to antifungal agents, which is further pronounced in the biofilm mode of Candida. Filamentation does not appear to be a major determinant of the antifungal resistance in Candida biofilms.

Original languageEnglish
Pages (from-to)333-339
Number of pages7
JournalInternational Journal of Antimicrobial Agents
Volume34
Issue number4
DOIs
Publication statusPublished - 2009 Oct
Externally publishedYes

Keywords

  • Antifungal susceptibility
  • Biofilm
  • Candida
  • Hyphal mutants

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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