Effect of fibronectin adsorption on osteoblastic cellular responses to hydroxyapatite and alumina

Masakazu Kawashita, Maki Hasegawa, Tada aki Kudo, Hiroyasu Kanetaka, Toshiki Miyazaki, Masami Hashimoto

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Initial cellular responses following implantation are important for inducing osteoconduction. We investigated cell adhesion, spreading, proliferation and differentiation of mouse MC3T3-E1 osteoblastic cells on untreated or fibronectin (Fn)-coated discs of hydroxyapatite (HAp) or alpha-type alumina (α-Al2O3). Fn coating significantly enhanced adhesion and spreading of MC3T3-E1 cells on HAp, but did not affect MC3T3-E1 cell proliferation and differentiation on HAp or α-Al2O3. Fn-coated HAp likely does not stimulate pre-osteoblast cells to initiate the process of osteoconduction; however, Fn adsorption might affect the response of inflammatory cells to the implanted material or, in conjunction with other serum proteins, stimulate pre-osteoblast cell proliferation and differentiation. Further studies on the effect of serum proteins in cell culture and the efficacy of Fn-coated HAp and α-Al2O3 in vivo are warranted.

Original languageEnglish
Pages (from-to)1268-1272
Number of pages5
JournalMaterials Science and Engineering C
Publication statusPublished - 2016 Dec 1


  • Alumina
  • Fibronectin
  • Hydroxyapatite
  • MC3T3-E1

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


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