Effect of fetal membrane-derived mesenchymal stem cell transplantation in rats with acute and chronic pancreatitis

Kazumichi Kawakubo, Shunsuke Ohnishi, Hirotoshi Fujita, Masaki Kuwatani, Reizo Onishi, Atsushi Masamune, Hiroshi Takeda, Naoya Sakamoto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objectives: Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis. Methods: Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCswere transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously. Results: The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC coculture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by tumor necrosis factor-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68- positive macrophages in the rat pancreas. The hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalPancreas
Volume45
Issue number5
DOIs
Publication statusPublished - 2016

Keywords

  • Acute pancreatitis
  • Amnion
  • Chronic pancreatitis
  • Fetal membrane
  • Macrophages
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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    Kawakubo, K., Ohnishi, S., Fujita, H., Kuwatani, M., Onishi, R., Masamune, A., Takeda, H., & Sakamoto, N. (2016). Effect of fetal membrane-derived mesenchymal stem cell transplantation in rats with acute and chronic pancreatitis. Pancreas, 45(5), 707-713. https://doi.org/10.1097/MPA.0000000000000541