Effect of exogenous salicylic acid supply on nodule formation of hypernodulating mutant and wild type of soybean

Takashi Sato, Hiroyuki Fujikake, Norikuni Ohtake, Kuni Sueyoshi, Tadashi Takahashi, Atsushi Sato, Takuji Ohyama

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Soybean plant is characterized by a systemic autoregulatory control system of nodulation (autoregulation) by initial infection with rhizobia, and plants commonly display a systemic acquired resistance (SAR) to pathogenic microbe infection related to salicylic acid (SA) signal transmission. We investigated the effect of exogenous SA supply on soybean nodulation to determine whether SA affects the autoregulation of nodulation. Seedlings of the hypernodulating mutants NOD1-3, NOD2-4 and their parent cv. Williams were treated or not treated (control) with a 100 μM-SA solution at 5 d before the inoculation of Bradyrhizobium japonicum strain USDA110. The nodule dry weight and the number of nodules of the wild type soybean Williams exhibiting autoregulation drastically decreased by the addition of 100 μM SA. The decrease in the nodule number was not caused by the reduction of the rhizobium number in the medium. Salicylic acid inhibited only early nodule formation and did not affect the growth of formed nodules. The inhibitory effect of SA on the nodulation of NOD1-3 and NOD2-4 was significantly less pronounced than that in Williams. These results indicate that SA is directly involved in signal transmission in the autoregulation, and that SA or the SAR induced by SA stimulates the autoregulation of nodulation in soybean.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalSoil Science and Plant Nutrition
Volume48
Issue number3
DOIs
Publication statusPublished - 2002 Jan 1

Keywords

  • Autoregulation
  • Hypernodulating mutant
  • Nodule
  • Salicylic acid
  • Soybean

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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