Effect of elimination of donor Kupffer cells and/or recipient macrophages on acute rejection in liver transplantation

Yorihiro Akamatsu, Nobuhiro Ohkohchi, Hideyuki Doi, Susumu Satomi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background/Aims: Kupffer cells/macrophages have pivotal roles of antigen presentation and cytokine production in the acute rejection after liver transplantation. In this study, we attempted to eliminate donor Kupffer cells and/or recipient macrophages by administration of liposome encapsulated LE-Cl2MDP (dichloromethylene diphosphonate) for clarification of the effect of depletion of Kupffer cells and macrophages on acute rejection. Methodology: To compare the survival, five groups were prepared: i) non-treatment group, ii) Kupffer cells eliminated group: transplanted Kupffer cells ehminated graft, iii) administration after liver transplantation group: injected LE-Cl2MDP twice after liver transplantation of non-treated graft, iv) additional administration group: transplanted Kupffer cells eliminated graft and injected LE-Cl2MDP twice after liver transplantation, and v) frequent administrations after liver transplantation group: injected LE-Cl2MDP every day for one week after liver transplantation of non-treated. Liver function tests, histological examination, histochemical staining of Kupffer cells and macrophages were carried out. Results: Survival days were not prolonged by Kupffer cells elimination of the graft or by depletion of migrated macrophages of recipient. In additional in the administration group, in which both Kupffer cells of the graft and migrated macrophages of recipient were eliminated, graft survival was prolonged significantly. But hyper bilirubinemia, hepatic fibrosis, and slight mononuclear cell infiltration were recognized. Conclusions: These results suggested that both donor Kupffer cells and recipient macrophages play important roles in acute rejection in liver transplantation.

Original languageEnglish
Pages (from-to)1105-1110
Number of pages6
JournalHepato-Gastroenterology
Volume50
Issue number52
Publication statusPublished - 2003 Jul

Keywords

  • Acute rejection
  • Kupffer cell
  • LE-ClMDP
  • Liver transplantation
  • Macrophage

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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