Effect of desensitizing agents on dentin permeability

Hiroshi Ishihata, Masafumi Kanehira, Tomoko Nagai, Werner J. Finger, Hidetoshi Shimauchi, Masashi Komatsu

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Purpose: To investigate the in vitro efficacy of two dentin desensitizing products at reducing liquid permeability through human dentin discs. The tested hypothesis was that the products, in spite of different chemical mechanisms were not different at reducing or eliminating flow through dentin discs. Methods: Dentin slices (1 mm thick) were prepared from 16 extracted human third molars and their permeability was indirectly recorded in a split chamber model, using a chemiluminescence technique, after EDTA treatment (control), after soaking with albumin, and after desensitizer application. Two products were studied: MS Coat, a self-curing resin-containing oxalate product, and Gluma Desensitizer, a glutaraldehyde/HEMA-based agent without initiator. The dentin slices were mounted between an upper chamber, filled with an aqueous solution of 1% potassium ferricyanide and 0.3% hydrogen peroxide, and a lower chamber filled with 1 % sodium hydroxide solution and 0.02% luminol. The upper solution was pressurized, and upon contact with the luminol solution a photochemical signal was generated and recorded as a measure of permeability throughout two consecutive pressurizing cycles at 2.5 and 13 kPa (26 and 133 cm H 2O), respectively. Results: The permeability of the control and albumin-soaked samples was similarly high. After application of the desensitizing agents, dentin permeability was reduced to virtually zero at both pressure levels (P< 0.001).

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalAmerican Journal of Dentistry
Volume22
Issue number3
Publication statusPublished - 2009 Jan 1

ASJC Scopus subject areas

  • Dentistry(all)

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