Effect of culture substrate surface on formation of embryoid body

Tomohiro Konno, Kunihiko Akita, Kimio Kurita, Yoshihiro Ito

Research output: Contribution to conferencePaper

Abstract

Mouse embryonic stem (ES) cells were cultured on a phospholipid polar groups-integrated surface (PC surface) and a conventional hydrophobic polystyrene surface called as bacterial culture grade Petri dish. ES cells were immediately aggregated on PC surface after seeding, and mono-aggregate (single embryoid body) was formed, and the size of embryoid body depended on the initial number of seeded ES cells. On polystyrene surface, the cells were locally aggregated, and the several aggregates were fused together. The formed embryoid body on PC surface highly expressed a transcription factor, CDH1, which was a marker of cell-cell adhesion rather than the embryoid body formed on polystyrene. On the other hand, the embryoid body formed on polystyrene highly expressed a transcription factor, Pax6, which was a marker of neural differentiation. It was concluded that the PC surface could induce the size-regulated embryoid body, and such embryoid body maintained the undifferentiated state.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Differentiation
  • Embryoid body
  • Embryonic stem cells
  • Phospholipid polymer surface
  • Undifferentiation

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Konno, T., Akita, K., Kurita, K., & Ito, Y. (2005). Effect of culture substrate surface on formation of embryoid body. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.