Effect of crosslinking agent in nanohydroxyapatite-collagen composite on ectopic bone formation with BMP-2 application

Taichi Tenkumo, Tsutomu Sugaya, Masamitsu Kawanami, Michael Gelinsky, Fumio Watari

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Histological investigations of the nanohydroxyapatite-collagen composite(nHAC) foil in tissue were carried out to evaluate its possible suitability as a BMP-2 carrier for regeneration of periodontal tissue. The nanohydroxyapatite-collagen composite and its thin foil could be successfully fabricated with the crosslinking agent of either 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide hydrochloride(EDC)or ascorbic acid / copper chloride (AA/CC). They were implanted into the subcutaneous tissue and tight muscle with and without BMP-2 for 1 and 2 weeks after surgery. nHAC was degraded and the inflammation in the surrounding area was decreased with time. nHAC(AA/CC) was degraded earlier and showed a better biocompatibility than nHAC(EDC). In the case of BMP-2 application, the different crosslinking agent showed an inverse effect. New bone formation was observed only in muscle for EDC and only in subcutaneous tissue for AA/CC. The Present results suggested that the nHAC is suitable as the carrier of BMP-2 with either the crosslinking agent of EDC or ascorbic acid / copper chloride, and the suitable crosslinking agent should be selected depending on desired conditions.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
JournalNano Biomedicine
Volume1
Issue number1
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • BMP-2
  • Hydroxyapatite
  • In vivo
  • Nanomaterials

ASJC Scopus subject areas

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Effect of crosslinking agent in nanohydroxyapatite-collagen composite on ectopic bone formation with BMP-2 application'. Together they form a unique fingerprint.

Cite this