Effect of chum salmon egg lectin on tight junctions in Caco-2 cell monolayers

Ryo Nemoto, Shintaro Yamamoto, Tomohisa Ogawa, Ryno Naude, Koji Muramoto

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER) value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca2+ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca2+, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine.

    Original languageEnglish
    Pages (from-to)8094-8106
    Number of pages13
    JournalMolecules
    Volume20
    Issue number5
    DOIs
    Publication statusPublished - 2015 May 1

    Keywords

    • Caco-2 cell
    • Intestinal transport
    • Lectin
    • Tight junction
    • Transepithelial transport

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Chemistry (miscellaneous)
    • Molecular Medicine
    • Pharmaceutical Science
    • Drug Discovery
    • Physical and Theoretical Chemistry
    • Organic Chemistry

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