Effect of Ca2+ on vesicle fusion on solid surface: An in vitro model of protein-accelerated vesicle fusion

Youichi Shinozaki, Ari M. Sittonen, Koji Sumitomo, Kazuaki Furukawa, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Lipid vesicle fusion is an important reaction in the cell. Calcium ions (Ca2+) participate in various important biological events including the fusion of vesicles with cell membranes in cells. We studied the effect of Ca2+ on the fusion of egg yolk phosphatidylcholine/brain phosphatidylserine (eggPC/brainPS) lipid vesicles on a mica substrate with fast scanning atomic force microscopy (AFM). When unattached and unfused lipid vesicles on mica were rinsed away, discrete patches of fused vesicles were observed under high Ca2+ concentrations. At 0 mM Ca2+, lipid vesicles were fused on mica and formed continuous supported lipid bilayers (SLBs) covering almost the entire mica surface. The effect of Ca2+ on SLB formation was offset by a Ca2+ chelating agent. When lipid vesicles were added during AFM observation, vesicles fused on mica and covered almost all areas even under high Ca2+ concentrations. These results indicate that force between AFM tip and vesicles overcomes the Ca 2+-reduced fusion of lipid vesicles.

Original languageEnglish
Pages (from-to)6164-6167
Number of pages4
JournalJapanese journal of applied physics
Volume47
Issue number7 PART 3
DOIs
Publication statusPublished - 2008 Jul 18
Externally publishedYes

Keywords

  • Calcium
  • Fast scanning AFM
  • Lipid
  • Supported lipid bilayer

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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