TY - JOUR
T1 - Effect of cadmium exposure on hepatopancreas and gills of the estuary mud crab (Scylla paramamosain)
T2 - Histopathological changes and expression characterization of stress response genes
AU - Zhu, Qi Hui
AU - Zhou, Zhong Kai
AU - Tu, Dan Dan
AU - Zhou, Yi Lian
AU - Wang, Cong
AU - Liu, Ze Peng
AU - Gu, Wen Bin
AU - Chen, Yu Yin
AU - Shu, Miao An
N1 - Funding Information:
This work was funded by Zhejiang Provincial Key Project of Science and Technology Research (No. 2015C02054 ), Zhejiang Provincial Key Project of Aquaculture New Varieties Breeding (No. 2016C02055-8 ), China Postdoctoral Science Foundation (No. 2016M601949 ).
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/2
Y1 - 2018/2
N2 - Cadmium (Cd) is a heavy metal that accumulates easily in organisms and causes several detrimental effects, including tissue damage. Cd contamination from anthropogenic terrestrial sources flows into rivers, and through estuaries to the ocean. To evaluate the toxic effects of Cd on estuary crustaceans, we exposed the mud crab Scylla paramamosain to various Cd concentrations (0, 10.0, 20.0, and 40.0 mg/L) for 24 h. We also exposed mud crabs to a fixed Cd concentration (20.0 mg/L) for various periods of time (0, 6, 12, 24, 48, and 72 h). We observed that after exposure to Cd, the surfaces of the gill lamellae were wrinkled, and the morphologies of the nuclei and mitochondria in the hepatopancreas were altered. We analyzed the expression profiles of 36 stress-related genes after Cd exposure, including those encoding metallothioneins, heat shock proteins, apoptosis-related proteins, and antioxidant proteins, with quantitative reverse transcription PCR. We found that exposure to Cd altered gene expression, and that some genes might be suitable bioindicators of Cd stress. Gene expression profiles were organ-, duration-, and concentration-dependent, suggesting that stress-response genes might be involved in an innate defense system for handling heavy metal exposure. To the best of our knowledge, this study is the first one of histopathology and stress-response gene expression pattern of Scylla paramamosain after Cd exposure. Our work could increase our understanding of the effect of environmental toxins on estuary crustaceans.
AB - Cadmium (Cd) is a heavy metal that accumulates easily in organisms and causes several detrimental effects, including tissue damage. Cd contamination from anthropogenic terrestrial sources flows into rivers, and through estuaries to the ocean. To evaluate the toxic effects of Cd on estuary crustaceans, we exposed the mud crab Scylla paramamosain to various Cd concentrations (0, 10.0, 20.0, and 40.0 mg/L) for 24 h. We also exposed mud crabs to a fixed Cd concentration (20.0 mg/L) for various periods of time (0, 6, 12, 24, 48, and 72 h). We observed that after exposure to Cd, the surfaces of the gill lamellae were wrinkled, and the morphologies of the nuclei and mitochondria in the hepatopancreas were altered. We analyzed the expression profiles of 36 stress-related genes after Cd exposure, including those encoding metallothioneins, heat shock proteins, apoptosis-related proteins, and antioxidant proteins, with quantitative reverse transcription PCR. We found that exposure to Cd altered gene expression, and that some genes might be suitable bioindicators of Cd stress. Gene expression profiles were organ-, duration-, and concentration-dependent, suggesting that stress-response genes might be involved in an innate defense system for handling heavy metal exposure. To the best of our knowledge, this study is the first one of histopathology and stress-response gene expression pattern of Scylla paramamosain after Cd exposure. Our work could increase our understanding of the effect of environmental toxins on estuary crustaceans.
KW - Cadmium
KW - Histopathological change
KW - Scylla paramamosain
KW - Stress response genes
UR - http://www.scopus.com/inward/record.url?scp=85036539831&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85036539831&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2017.11.020
DO - 10.1016/j.aquatox.2017.11.020
M3 - Article
C2 - 29197714
AN - SCOPUS:85036539831
VL - 195
SP - 1
EP - 7
JO - Aquatic Toxicology
JF - Aquatic Toxicology
SN - 0166-445X
ER -