Effect of cadmium exposure on hepatopancreas and gills of the estuary mud crab (Scylla paramamosain): Histopathological changes and expression characterization of stress response genes

Qi Hui Zhu, Zhong Kai Zhou, Dan Dan Tu, Yi Lian Zhou, Cong Wang, Ze Peng Liu, Wen Bin Gu, Yu Yin Chen, Miao An Shu

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Cadmium (Cd) is a heavy metal that accumulates easily in organisms and causes several detrimental effects, including tissue damage. Cd contamination from anthropogenic terrestrial sources flows into rivers, and through estuaries to the ocean. To evaluate the toxic effects of Cd on estuary crustaceans, we exposed the mud crab Scylla paramamosain to various Cd concentrations (0, 10.0, 20.0, and 40.0 mg/L) for 24 h. We also exposed mud crabs to a fixed Cd concentration (20.0 mg/L) for various periods of time (0, 6, 12, 24, 48, and 72 h). We observed that after exposure to Cd, the surfaces of the gill lamellae were wrinkled, and the morphologies of the nuclei and mitochondria in the hepatopancreas were altered. We analyzed the expression profiles of 36 stress-related genes after Cd exposure, including those encoding metallothioneins, heat shock proteins, apoptosis-related proteins, and antioxidant proteins, with quantitative reverse transcription PCR. We found that exposure to Cd altered gene expression, and that some genes might be suitable bioindicators of Cd stress. Gene expression profiles were organ-, duration-, and concentration-dependent, suggesting that stress-response genes might be involved in an innate defense system for handling heavy metal exposure. To the best of our knowledge, this study is the first one of histopathology and stress-response gene expression pattern of Scylla paramamosain after Cd exposure. Our work could increase our understanding of the effect of environmental toxins on estuary crustaceans.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalAquatic Toxicology
Volume195
DOIs
Publication statusPublished - 2018 Feb
Externally publishedYes

Keywords

  • Cadmium
  • Histopathological change
  • Scylla paramamosain
  • Stress response genes

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

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