Effect of biotin treatment on hepatic gene expression in streptozotocin-induced diabetic rats

Yumi Sugita, Hitoshi Shirakawa, Ritsuko Sugimoto, Yuji Furukawa, Michio Komai

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Biotin functions as a coenzyme for four carboxylases involved in energy metabolism in mammals. Besides these classical functions, biotin has novel functions in the cellular processes via the modulation of gene expression. In this study, we examined the alteration of gene expression by biotin administration in the liver of streptozotocin (STZ)-induced diabetic rats. In comparison with the control, the mRNA levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were significantly reduced and glucokinase mRNA was increased 3 h after the administration of biotin or insulin. The expression of hepatocyte nuclear factor 4α, one of the transcription factors responsible for gluconeogenic gene expression, was decreased by biotin at both mRNA and protein levels. In addition, forkhead box O1 and sterol regulatory element-binding protein 1c mRNA expression that was enhanced by the insulin treatment was inversely decreased by biotin. These results indicate that biotin repressed the gluconeogenic genes and their transcription factors via a pathway independent of insulin-signaling and could improve the diabetic condition.

    Original languageEnglish
    Pages (from-to)1290-1298
    Number of pages9
    JournalBioscience, Biotechnology and Biochemistry
    Volume72
    Issue number5
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Biotin
    • Gene expression
    • Gluconeogenesis
    • Streptozotocin (STZ) diabetic rat

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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