Effect of Astaxanthin from Phaffia Rhodozymct on T cell Population and Responsiveness to Mitogen in Splenic Mononuclear Cells of Male Broiler Chicks

Tetsuya Takimoto, Kan Sato, Yukio Akiba

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Effect of feeding astaxanthin (Ax) provided as Phaffia rhodozyma on T cell population and responsiveness to mitogen, concanavalin A (Con A) or phytohemaggulutinin (PHA), of splenic mononuclear cells (MNC) was determined in male broiler chicks. Chicks at1 week of age, were fed ad libitum on diets containing 0, 20 or 100 ppm Ax for 7 days. Feeding the diet containing Ax decreased percentage of CD3-positive cells, an indicator of the T cell population in splenic lymphocytes. Feeding the diet containing 20 ppm Ax decreased percentage of CD4-positive cells estimated as the indicator of helper T cells, but no modulation was observed by feeding the diet containing100 ppm Ax. Percentage of CD8-positive cells, an indicator of cytotoxic T cell, was not affected by feeding diets containing either 20 or 100 ppm Ax. T cell proliferation stimulated with Con A was enhanced by feeding diet containing 20 ppm Ax, but not affected by 100 ppm Ax. Feeding diets containing 20 and 100 ppm Ax enhanced the proliferation of splenocytes against PHA. Feeding diets containing Ax showed no effect on interleukin(IL)-2mRNA expression in splenocytes stimulated by Con A estimated as the T cell proliferation promoting factor. IL-2 like activity was slightly, but significantly, decreased by feeding diet containing 20 ppm Ax. IFN-γmRNA expression, estimated as a T cell activator under condition of Con A stimulation, was higher in chicks fed the diet containing 20 ppm Ax. These results suggest that proliferation and cytokine expressions of splenic T cells in response to feeding Phaffia rhodozyma containing Ax were independent of the dietary Ax concentration or total Ax intake. The enhancement of T cell proliferation by feeding 20ppm Ax might be caused by changes in IFN-γ rather than IL-2 production in the chick splenocytes.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalJournal of Poultry Science
Volume43
Issue number1
DOIs
Publication statusPublished - 2006

Keywords

  • Phaffia yeast
  • T cell population
  • T cell responsiveness
  • astaxanthin
  • chicken splenocytes

ASJC Scopus subject areas

  • Animal Science and Zoology

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