Effect of antibiotics, levofloxacin and fosfomycin, on a mouse model with Escherichia coli O157 infection

Emiko Isogai, Hiroshi Isogai, Shunji Hayashi, Toru Kubota, Koichi Kimura, Nobuhiro Fujii, Tsuyoshi Ohtani, Kenichi Sato

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

There have been some reservations about the treatment of enterohemorrhagic Escherichia coli (EHEC) infection with antibiotics to prevent the occurrence of hemolytic uremic syndrome (HUS). However, the administration of antimicrobial agents for EHEC infection is under discussion. Therefore, we used an experimental mouse model to assess the advantage/disadvantage of two major antibiotics, levofloxacin (LVFX) and fosfomycin (FOM). Germ-free IQI mice were inoculated with EHEC O157 strain EDL931 or 7. Bacteria colonized feces at 109-1010 CFU/g, and Shiga toxins (STXs) were detected in the feces. From 1 day after infection, mice were assigned to LVFX (20 mg/kg) once daily or FOM (400 mg/kg) once daily. A significant decrease in overall mortality was observed after treatment of LVFX, with EHEC disappearing immediately from the feces of mice. FOM also reduced mortality for one strain, the STX level decreased gradually. LVFX exhibited higher therapeutic efficacy than FOM. Strain differences were observed in the model during the treatment.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number2
DOIs
Publication statusPublished - 2000

Keywords

  • Escherichia coli O157
  • Fosfomycin
  • Levofloxacin
  • Mouse model

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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