Effect of an external electric field on the kinetics of dislocation-free growth of tetragonal hen egg white lysozyme crystals

Haruhiko Koizumi, Satoshi Uda, Kozo Fujiwara, Junpei Okada, Jun Nozawa

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Dislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates of the crystal measured under an applied field at 1 MHz was observed, although the overall driving force increased. Assuming that the birth and spread mechanism of two-dimensional nucleation occurs, an increase in the effective surface energy of the step ends was realized in the presence of the electric field, which led to an improvement in the crystal quality of the tetragonal HEW lysozyme crystals. This article also discusses the increase in the effective surface energy of the step ends with respect to the change in the entropy of the solid.

Original languageEnglish
Article number170
JournalCrystals
Volume7
Issue number6
DOIs
Publication statusPublished - 2017 Jun 10

Keywords

  • Crystal quality
  • Electric field
  • Growth kinetics
  • Protein crystals

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Effect of an external electric field on the kinetics of dislocation-free growth of tetragonal hen egg white lysozyme crystals'. Together they form a unique fingerprint.

Cite this