Effect of acoustic overstimulation on regulation of glucocorticoid receptor mRNA in the cochlea of the guinea pig

Tsumoru Terunuma, Akira Hara, Masamitsu Senarita, Hozumi Motohashi, Jun Kusakari

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Semi-quantitative reverse transcription polymerase chain reaction was performed to determine the distribution of mRNA levels of glucocorticoid receptor (GR) within the guinea pig cochlea and to examine the change in their expression after acoustic overstimulation. Using an original PCR primer for the guinea pig, the highest GR mRNA level was revealed in the modiolus and lowest in the medial portion including the organ of Corti. Total RNA was extracted from the whole cochlea of the guinea pig 0, 2, 6 and 24 h after exposure to a 2 kHz pure tone of 110, 120 or 130 dB SPL for 10 min. The level of GR mRNA significantly decreased immediately and 2 h after exposure to the sound of 120 dB SPL, and 2 and 6 h after exposure to that of 130 dB SPL. These results suggest the presence of a down-regulation of GR mRNA induced by acoustic overstimulation, although the exact mechanism of this phenomenon remains unsolved. Copyright (C) 2001 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalHearing Research
Volume151
Issue number1-2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Acoustic overstimulation
  • Glucocorticoid receptor
  • mRNA

ASJC Scopus subject areas

  • Sensory Systems

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