Effect of a topical steroid on gene expressions for chemokines in mice with contact hypersensitivity

Gaku Mitsui, Takeo Hirano, Yoshimi Niwano, Kazutaka Mitsui, Osamu Ohara, Satoshi Yanagihara, Masatoshi Kato

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Effects of a topical corticosteroid drug, diflucortolone valerate, on the mRNA expressions for four CC- and four CXC-chemokines, which have been reported to be associated with recruitment of different kinds of proinflammatory and inflammatory cells, were investigated by RT-PCR in mice with 2,4,6-trinitrochlorobenzene (TNCB)-induced contact hypersensitivity (CHS) response. All of the eight gene expressions were clearly up-regulated in the lesion site of the CHS response up to 24 h post-challenge of TNCB at which ear swelling response reached a peak, so that heavy infiltration of inflammatory cells consisting mainly of mononuclear cells and neutrophils was likely induced by these chemokines. Topical treatment with diflucortolone valerate suppressed completely the infiltrates as well as the ear swelling response. In addition, the up-regulation of gene expressions for these eight chemokines were suppressed by the treatment, indicating that the corticosteroid drug attenuates the expression of chemokine genes essential for orientating nonspecific skin response to hapten-specific CHS response through the recruitment of inflammatory cells from the circulation into the tissue site.

Original languageEnglish
Pages (from-to)57-69
Number of pages13
JournalInternational Immunopharmacology
Volume4
Issue number1
DOIs
Publication statusPublished - 2004 Jan
Externally publishedYes

Keywords

  • Chemokine genes
  • Contact hypersensitivity
  • Corticosteroid
  • RT-PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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