TY - JOUR
T1 - Effect of 5-fluoro-2'-deoxyuridine on toxin production and cell cycle regulation in marine dinoflagellate, Alexandrium tamarense
AU - Cho, Yuko
AU - Ogawa, Motoo
AU - Yotsu-Yamashita, Mari
AU - Oshima, Yasukatsu
N1 - Funding Information:
This work was supported by KAKENHI Grant Number 21658075 and 24580295 from the Japanese Society for the Promotion of Science (JSPS) awarded to Y. Cho and the Funding Program for the Next Generation World-Leading Researchers (LS012) awarded to M. Yotsu-Yamashita from JSPS. We thank Dr. T. Omura, who kindly provided A. tamarense clonal culture strain Axat-2.[SS]
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2014/2
Y1 - 2014/2
N2 - The effect of metabolic inhibitor, 5-fluoro-2'-deoxyuridine (FUdR) on toxin production and the cell cycle of marine dinoflagellate, Alexandrium tamarense, was investigated. Compared to untreated cells, FUdR at 3μM (p<0.05) to 300μM (p<0.01) inhibited the cell proliferation and toxin production in a dose-dependent manner for A. tamarense cultured in modified T1 medium. FUdR at 203μM resulted in cell cycle arrest at the S phase at day 4 and toxigenesis was inhibited after day 2. The toxin profiles of the FUdR-treated cultures were similar to those of the control culture. These results suggest that FUdR inhibits saxitoxin (STX) biosynthesis in the early stage of the pathway. This report is the first to demonstrate the inhibition of toxin production in A. tamarense by a nucleoside analog.
AB - The effect of metabolic inhibitor, 5-fluoro-2'-deoxyuridine (FUdR) on toxin production and the cell cycle of marine dinoflagellate, Alexandrium tamarense, was investigated. Compared to untreated cells, FUdR at 3μM (p<0.05) to 300μM (p<0.01) inhibited the cell proliferation and toxin production in a dose-dependent manner for A. tamarense cultured in modified T1 medium. FUdR at 203μM resulted in cell cycle arrest at the S phase at day 4 and toxigenesis was inhibited after day 2. The toxin profiles of the FUdR-treated cultures were similar to those of the control culture. These results suggest that FUdR inhibits saxitoxin (STX) biosynthesis in the early stage of the pathway. This report is the first to demonstrate the inhibition of toxin production in A. tamarense by a nucleoside analog.
KW - 5-Fluoro-2'-deoxyuridine (FUdR)
KW - Alexandrium tamarense
KW - Paralytic shellfish toxins
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U2 - 10.1016/j.hal.2013.12.007
DO - 10.1016/j.hal.2013.12.007
M3 - Article
AN - SCOPUS:84892523174
SN - 1568-9883
VL - 32
SP - 64
EP - 72
JO - Harmful Algae
JF - Harmful Algae
ER -
