Effect of 1,25(OH)2D3 on expression of estrogen receptor-alpha mRNA on rat osteosarcoma cell line (ROS 17/2.8)

Minoru Ohsawa, Hideki Mizunuma, Issei Kagami, Sumitaka Miyamoto, Tatsuya Kanuma, Yoshito Ibuki

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In order to investigate the regulatory mechanisms of estrogen receptors (ER) in bone cells, changes in ER-alpha mRNA levels of rat osteosarcoma cell line (ROS 17/2.8) before and after exposure to 1,25(OH)2D3 and 17-beta estradiol respectively were measured by quantitative polymerase chain reaction using an internal standard. ER mRNA levels in the ROS 17/2.8 cultured with the medium alone had 5.029 ±1.623 mol/g total RNA x 10-13 and were not statistically different from those cultured in the presence of 1,25(OH)2D3 at concentrations of 10-12 M and less. ER mRNA levels in the ROS 17/2.8 cell line showed a small but a significant increase as a result of stimulation by 1,25(OH)2D3 at concentrations of 10-10 and 10-11 M. However, ER mRNA levels in ROS 17/2.8 cultured in the presence of 1,25(OH)2D3 at concentrations of 10-9 M were not statistically different from those of the control. On the other hand, the expression of ER in ROS 17/2.8 cells cultured for 3 hours with various doses of 1,25(OH)2D3 showed, by immunoblotting methods, a significant increase at the dose of 10-10 M in the expression of ER. Although a physiological significance is obscure, these observations suggest that 1,25(OH)2D3 plays a part in the expression of ER in ROS 17/2.8. No significant changes were seen in the expression of ER mRNA and the synthesis of ER as a result of stimulation by the estradiol.

Original languageEnglish
Pages (from-to)2465-2472
Number of pages8
JournalLife Sciences
Volume66
Issue number25
DOIs
Publication statusPublished - 2000 May 12
Externally publishedYes

Keywords

  • 1,25(OH)D
  • ER mRNA
  • Estrogen
  • Estrogen receptors
  • Quantitative PCR
  • ROS 17/2.8

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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