EDTA-FISH: A simple and effective approach to reduce non-specific adsorption of probes in fluorescence in situ hybridization (FISH) for environmental samples

Yuki Morono, Kengo Kubota, Daisuke Tsukagoshi, Takeshi Terada

Research output: Contribution to journalArticlepeer-review

Abstract

Fluorescence in situ hybridization (FISH) is a widely used molecular technique in microbial ecology. However, the non-specific adsorption of fluorescent probes and resulting high intensity of background signals from mineral particles hampers the specific detection of microbial cells in grain-rich environmental samples, such as subseafloor sediments. We herein demonstrated that a new buffer composition containing EDTA efficiently reduced the adsorption of probes without compromising the properties of the FISH-based probing of microbes. The inclusion of a high concentration of EDTA in the buffer in our protocol provides a simple and effective approach for reducing the background in FISH for environmental samples.

Original languageEnglish
Article numberME20062
Pages (from-to)1-4
Number of pages4
JournalMicrobes and environments
Volume35
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • EDTA
  • Fluorescence in situ hybridization (FISH)
  • Marine sediments

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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