Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations

N. Sasano; A. Enomoto; Y. Hosoi; Y. Katsumura; Y. Matsumoto; A. Morita; K. Shiraishi; K. Miyagawa; H. Igaki; K. Nakagawa

doi:10.1016/j.canlet.2009.12.020

Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations

N. Sasano, A. Enomoto, Y. Hosoi, Y. Katsumura, Y. Matsumoto, A. Morita, K. Shiraishi, K. Miyagawa, H. Igaki, K. Nakagawa

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Edaravone has been reported to have a radioprotective effect at high concentrations. We now report that a lower dose of edaravone enhanced X-ray-induced apoptosis of some cell lines harboring p53 wild-type status, such as MOLT-4, Nalm-6, and HepG2. The knock-down of p53 using siRNA in MOLT-4 cells abolished the radiosensitizing effect of edaravone. Enhanced phosphorylations of p53 at Ser 15 and Ser 20 and up-regulation of PUMA, a p53 target protein, were observed after X-irradiation in the presence of edaravone. We conclude that the low dose of edaravone sensitized cells to X-irradiation by promoting the p53-dependent apoptotic signaling pathway.

Original language	English
Pages (from-to)	52-57
Number of pages	6
Journal	Cancer Letters
Volume	293
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2009.12.020
Publication status	Published - 2010 Jul
Externally published	Yes

Keywords

Apoptosis
Edaravone
P53
Radiosensitizer

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2009.12.020

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@article{55673b28e89340a8bec8c7994bbaee86,

title = "Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations",

abstract = "Edaravone has been reported to have a radioprotective effect at high concentrations. We now report that a lower dose of edaravone enhanced X-ray-induced apoptosis of some cell lines harboring p53 wild-type status, such as MOLT-4, Nalm-6, and HepG2. The knock-down of p53 using siRNA in MOLT-4 cells abolished the radiosensitizing effect of edaravone. Enhanced phosphorylations of p53 at Ser 15 and Ser 20 and up-regulation of PUMA, a p53 target protein, were observed after X-irradiation in the presence of edaravone. We conclude that the low dose of edaravone sensitized cells to X-irradiation by promoting the p53-dependent apoptotic signaling pathway.",

keywords = "Apoptosis, Edaravone, P53, Radiosensitizer",

author = "N. Sasano and A. Enomoto and Y. Hosoi and Y. Katsumura and Y. Matsumoto and A. Morita and K. Shiraishi and K. Miyagawa and H. Igaki and K. Nakagawa",

note = "Funding Information: We thank all the members of our laboratory for their help and encouragement. We also thank Dr. T. Kondo (University of Toyama) for various suggestions and instructions. This study was supported in part by the Mitsubishi Tanabe Pharma Corporation (Tokyo, Japan). This study was also supported in part by the Radiation Effects Association.",

year = "2010",

month = jul,

doi = "10.1016/j.canlet.2009.12.020",

language = "English",

volume = "293",

pages = "52--57",

journal = "Cancer Letters",

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T1 - Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations

AU - Sasano, N.

AU - Enomoto, A.

AU - Hosoi, Y.

AU - Katsumura, Y.

AU - Matsumoto, Y.

AU - Morita, A.

AU - Shiraishi, K.

AU - Miyagawa, K.

AU - Igaki, H.

AU - Nakagawa, K.

N1 - Funding Information: We thank all the members of our laboratory for their help and encouragement. We also thank Dr. T. Kondo (University of Toyama) for various suggestions and instructions. This study was supported in part by the Mitsubishi Tanabe Pharma Corporation (Tokyo, Japan). This study was also supported in part by the Radiation Effects Association.

PY - 2010/7

Y1 - 2010/7

N2 - Edaravone has been reported to have a radioprotective effect at high concentrations. We now report that a lower dose of edaravone enhanced X-ray-induced apoptosis of some cell lines harboring p53 wild-type status, such as MOLT-4, Nalm-6, and HepG2. The knock-down of p53 using siRNA in MOLT-4 cells abolished the radiosensitizing effect of edaravone. Enhanced phosphorylations of p53 at Ser 15 and Ser 20 and up-regulation of PUMA, a p53 target protein, were observed after X-irradiation in the presence of edaravone. We conclude that the low dose of edaravone sensitized cells to X-irradiation by promoting the p53-dependent apoptotic signaling pathway.

AB - Edaravone has been reported to have a radioprotective effect at high concentrations. We now report that a lower dose of edaravone enhanced X-ray-induced apoptosis of some cell lines harboring p53 wild-type status, such as MOLT-4, Nalm-6, and HepG2. The knock-down of p53 using siRNA in MOLT-4 cells abolished the radiosensitizing effect of edaravone. Enhanced phosphorylations of p53 at Ser 15 and Ser 20 and up-regulation of PUMA, a p53 target protein, were observed after X-irradiation in the presence of edaravone. We conclude that the low dose of edaravone sensitized cells to X-irradiation by promoting the p53-dependent apoptotic signaling pathway.

KW - Apoptosis

KW - Edaravone

KW - P53

KW - Radiosensitizer

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Edaravone, a known free radical scavenger, enhances X-ray-induced apoptosis at low concentrations

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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