TY - JOUR
T1 - Ectopic transcription and the possibility of RNA editing of the human arrestin gene
AU - Wada, Yuko
AU - Nakazawa, Mitsuru
AU - Abe, Toshiaki
AU - Tamai, Makoto
N1 - Funding Information:
This study was supported in part by a grant (to Dr. Nakazawa) from the Research Committee on Chorioretinal Degeneration and Optic Atrophy, the Ministry of Health and Welfare of the Japanese Government, Tokyo, Japan, and a grant-in-aid (to Dr. Nakazawa, C-2-09671782) for scientific research from the Ministry of Education, Science, Sports and Culture of the Japanese Government, Tokyo, Japan.
PY - 1999/7
Y1 - 1999/7
N2 - Purpose: To investigate the ectopic transcription of arrestin in various human organs and tissues.Methods: Reverse transcriptase-polymerase chain reaction was used to investigate the ectopic transcription of the arrestin gene in the retina, anterior capsule of the lens, iris, bulbar conjunctiva, muscle, skin, placenta, intestine, and human blood. In addition, we examined the expression of arrestin mRNA isolated from whole blood cells of patients with Oguchi disease who had the 1147delA mutation in the arrestin gene.Results: Arrestin was expressed at the mRNA level in all our samples. The mRNA isolated from the blood cells of Oguchi patients was not associated with the same 1147delA mutation as was reported earlier in the arrestin gene but had the normal sequence.Conclusions: These results demonstrate the ectopic transcription of the arrestin gene in various tissues and also suggest the possibility of RNA editing in the blood cells of Oguchi patients. Copyright (C) 1999 Japanese Ophthalmological Society.
AB - Purpose: To investigate the ectopic transcription of arrestin in various human organs and tissues.Methods: Reverse transcriptase-polymerase chain reaction was used to investigate the ectopic transcription of the arrestin gene in the retina, anterior capsule of the lens, iris, bulbar conjunctiva, muscle, skin, placenta, intestine, and human blood. In addition, we examined the expression of arrestin mRNA isolated from whole blood cells of patients with Oguchi disease who had the 1147delA mutation in the arrestin gene.Results: Arrestin was expressed at the mRNA level in all our samples. The mRNA isolated from the blood cells of Oguchi patients was not associated with the same 1147delA mutation as was reported earlier in the arrestin gene but had the normal sequence.Conclusions: These results demonstrate the ectopic transcription of the arrestin gene in various tissues and also suggest the possibility of RNA editing in the blood cells of Oguchi patients. Copyright (C) 1999 Japanese Ophthalmological Society.
KW - 1147delA mutation
KW - Arrestin gene
KW - Ectopic transcription
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U2 - 10.1016/S0021-5155(99)00022-2
DO - 10.1016/S0021-5155(99)00022-2
M3 - Article
C2 - 10482475
AN - SCOPUS:0032800707
VL - 43
SP - 295
EP - 299
JO - Japanese Journal of Ophthalmology
JF - Japanese Journal of Ophthalmology
SN - 0021-5155
IS - 4
ER -
