Ectopic bone formation induced by biodegradable hydrogels incorporating bone morphogenetic protein)?

Masaya Yamamoto, Yasuhiko Tabata, Yoshito Ikada

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68 Citations (Scopus)


Biodegradable hydrogels were prepared from gelatin by glutaraldehyde cross-linking for re- lease matrix of recombinant human bone morphogenetic protein-2 (BMP-2). BMP-2 solution was im- pregnated into the dried hydrogels to prepare BMP-2-incorporating gelatin hydrogels. In the in vitro study, enhanced retention of BMP-2 was observed from the BMP-2-incorporating gelatin hydrogels after an initial burst of BMP-2 incorporated initially in the hydrogel. Following subcutaneous implantation of 125I-labeled BMP-2-incorporating gelatin hydrogels in the back of mice, the radioactivity remaining in the hydrogels was measured to estimate the in vivo release profile of BMP-2. It was found that BMP-2 was retained in the hydrogels for longer than 30 days, whereas 99% of BMP-2 injected in the solution form was cleared from the injected site within one day, completely disappearing within 3 days. Ectopic bone formation studies demonstrated that BMP-2-incorporating gelatin hydrogels exhibited a more potent ability for bone induction than solution injection of BMP-2. This finding indicates that enhanced retention of BMP-2 is promotes its ability to induce ectopic bone formation.

Original languageEnglish
Pages (from-to)439-458
Number of pages20
JournalJournal of Biomaterials Science, Polymer Edition
Issue number5
Publication statusPublished - 1998 Jan 1
Externally publishedYes


  • Biodegradable hydrogel
  • Ectopic bone formation
  • Gelatin
  • Recombinant human BMP-2
  • Release matrix

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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