Ecm10p localizes in yeast mitochondrial nucleoids and its overexpression induces extensive mitochondrial DNA aggregations

Yuji Sakasegawa, Naomi S. Hachiya, Shoichiro Tsukita, Kiyotoshi Kaneko

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Ecm10p was initially identified as a cell wall synthesis-related gene product [Genetics 147 (1997) 435] and also reported as a mitochondrial protein which was partially capable of compensating the phenotypic defect by SSC1 gene mutation [FEBS Lett. 487 (2000) 307]. Here we report that ecm10p is localized in mitochondrial nucleoids as its major component and the targeting signal resides between amino acid residues 161 and 240. Overexpression of ecm10p induces extensive mitochondrial DNA aggregations, which might be due to aberrant mitochondrial DNA cleavages through an altered endonuclease activity in mitochondrial nucleoids.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalBiochemical and biophysical research communications
Volume309
Issue number1
DOIs
Publication statusPublished - 2003 Sep 12

Keywords

  • Heat-shock protein
  • Mitochondrial DNA
  • Mitochondrial nucleoids
  • Saccharomyces cerevisiae
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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