Early postoperative nociceptive threshold and production of brain derived neurotrophic factor induced by plantar incision are not influenced with minocycline in a rat: Role of Spinal microglia

Eiji Masaki, Kentaro Mizuta, Norimasa Ohtani, Kanta Kido

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background: Brain-derived neurotrophic factor (BDNF) from spinal microglia is crucial for aberrant nociceptive signaling in several pathological pain conditions, including postoperative pain. We assess the contribution of spinal microglial activation and associated BDNF overexpression to the early post-incisional nociceptive threshold. Methods: Male SpragueDawley rats were implanted with an intrathecal catheter. A postoperative pain model was established by plantar incision. Thermal and mechanical nociceptive responses were assessed by infrared radiant heat and von Frey flaments before and after plantar incision. Rats were injected intrathecally the microglial activation inhibitor minocycline before incision, 24 h after incision, or both. Other groups were subjected to the same treatments and the L4-L5 spinal cord segment removed for immunohistochemical analysis of microglia activation and BNDF expression. Results: Plantar incision reduced both thermal latency and mechanical threshold, indicating thermal hypersensitivity and mechanical allodynia. Minocycline temporally reduced thermal withdrawal latency but had no effect on mechanical withdrawal threshold, spinal microglial activity, or dorsal horn BDNF overexpression during the early post-incision period. Conclusion: These results suggest that spinal microglia does not contribute substantially to post-incisional nociceptive threshold. The BDNF overexpression response that may contribute to postoperative hyperalgesia and allodynia is likely derived from other sources.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
JournalNeuroSignals
Volume24
Issue number1
DOIs
Publication statusPublished - 2016

Keywords

  • Brain-derived neurotrophic factor
  • Early postoperative pain
  • Microglia
  • Spinal cord

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

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