TY - JOUR
T1 - Dynein-Mediated Regional Cell Division Reorientation Shapes a Tailbud Embryo
AU - Nakamoto, Ayaki
AU - Kumano, Gaku
N1 - Funding Information:
We thank Drs. Shigeo Hayashi and Kagayaki Kato for their technical advices for live imaging; Dr. Anna Di Gregorio for the Tbx6b >PH-YFP construct; Dr. Alex McDougall for the MAP7-GFP and PH-dTomato constructs; Dr. Takefumi Negishi for H2B-GFP construct and helpful discussion. This study was supported by The Sumitomo Foundation (G.K.), Yamada Science Foundation (G.K.), and Grant-in-Aid for Scientific Research (KAKENHI) on Innovative Areas ( 23111515 to G.K.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan . Also, this study was supported by Narishige Zoological Science Award (A.N.), and Grant-in-Aid for Scientific Research (KAKENHI) ( 19K06735 to A.N.), Japan Society for the Promotion of Science .
PY - 2020/3/27
Y1 - 2020/3/27
N2 - Regulation of cell division orientation controls the spatial distribution of cells during development and is essential for one-directional tissue transformation, such as elongation. However, little is known about whether it plays a role in other types of tissue morphogenesis. Using an ascidian Halocynthia roretzi, we found that differently oriented cell divisions in the epidermis of the future trunk (anterior) and tail (posterior) regions create an hourglass-like epithelial bending between the two regions to shape the tailbud embryo. Our results show that posterior epidermal cells are polarized with dynein protein anteriorly localized, undergo dynein-dependent spindle rotation, and divide along the anteroposterior axis. This cell division facilitates constriction around the embryo's circumference only in the posterior region and epithelial bending formation. Our findings, therefore, provide an important insight into the role of oriented cell division in tissue morphogenesis.
AB - Regulation of cell division orientation controls the spatial distribution of cells during development and is essential for one-directional tissue transformation, such as elongation. However, little is known about whether it plays a role in other types of tissue morphogenesis. Using an ascidian Halocynthia roretzi, we found that differently oriented cell divisions in the epidermis of the future trunk (anterior) and tail (posterior) regions create an hourglass-like epithelial bending between the two regions to shape the tailbud embryo. Our results show that posterior epidermal cells are polarized with dynein protein anteriorly localized, undergo dynein-dependent spindle rotation, and divide along the anteroposterior axis. This cell division facilitates constriction around the embryo's circumference only in the posterior region and epithelial bending formation. Our findings, therefore, provide an important insight into the role of oriented cell division in tissue morphogenesis.
KW - Developmental Biology
KW - Embryology
KW - Organizational Aspects of Cell Biology
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U2 - 10.1016/j.isci.2020.100964
DO - 10.1016/j.isci.2020.100964
M3 - Article
AN - SCOPUS:85081659776
VL - 23
JO - iScience
JF - iScience
SN - 2589-0042
IS - 3
M1 - 100964
ER -