Dynamin is primed at endocytic sites for ultrafast endocytosis

Yuuta Imoto, Sumana Raychaudhuri, Ye Ma, Pascal Fenske, Eduardo Sandoval, Kie Itoh, Eva Maria Blumrich, Hideaki T. Matsubayashi, Lauren Mamer, Fereshteh Zarebidaki, Berit Söhl-Kielczynski, Thorsten Trimbuch, Shraddha Nayak, Janet H. Iwasa, Jian Liu, Bin Wu, Taekjip Ha, Takanari Inoue, Erik M. Jorgensen, Michael A. CousinChristian Rosenmund, Shigeki Watanabe

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons internalizes vesicles as quickly as 50 ms during synaptic vesicle recycling. Here, we demonstrate that Dynamin 1 is pre-recruited to endocytic sites for ultrafast endocytosis. Specifically, Dynamin 1xA, a splice variant of Dynamin 1, interacts with Syndapin 1 to form molecular condensates on the plasma membrane. Single-particle tracking of Dynamin 1xA molecules confirms the liquid-like property of condensates in vivo. When Dynamin 1xA is mutated to disrupt its interaction with Syndapin 1, the condensates do not form, and consequently, ultrafast endocytosis slows down by 100-fold. Mechanistically, Syndapin 1 acts as an adaptor by binding the plasma membrane and stores Dynamin 1xA at endocytic sites. This cache bypasses the recruitment step and accelerates endocytosis at synapses.

Original languageEnglish
Pages (from-to)2815-2835.e13
JournalNeuron
Volume110
Issue number17
DOIs
Publication statusPublished - 2022 Sept 7
Externally publishedYes

Keywords

  • Dyn1xA
  • Dynamin
  • Dynamin splice variants
  • Syndapin
  • endocytosis
  • flash-and-freeze
  • liquid condensates
  • phase separation
  • synaptic vesicle recycling
  • ultrafast endocytosis

ASJC Scopus subject areas

  • Neuroscience(all)

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